Effects of dietary quercetin on retrieved mouse oocytes and in vitro fertilization outcomes.

IF 1.8 Q3 OBSTETRICS & GYNECOLOGY

Jornal Brasileiro de Reproducao Assistida Pub Date : 2024-11-14 DOI:10.5935/1518-0557.20240073

Rasrawee Chantrasiri, Pannarai Somboonchai, Waraporn Piromlertamorn, Tawiwan Pantasri, Usanee Sanmee

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Abstract

Objective: To investigate the effects of dietary quercetin on the retrieved mouse oocytes and IVF outcomes.

Methods: Female mice were divided into two groups. Mice were given 0.2 mL water without (control group) or with quercetin 30 mg/kg (quercetin group) orally via gavage, once a day for 21 consecutive days. After that female mice were superovulated for in vitro fertilization. We observed the effect of dietary quercetin on the number of retrieved oocytes, oocyte degeneration rate, fertilization rate, blastocyst formation rate, and blastocyst cell numbers.

Results: There was no difference in the number of retrieved oocytes per mouse (27.3±6.7 and 27.2±5.8), oocyte fragmentation rate (28.4% and 25.0%), fertilization rate (47.4% and 50.6%) and blastocyst formation rate (34.8% and 34.7%) in the quercetin group compared to the control group. The proportion of hatching and hatched blastocyst was significantly lower in the quercetin group (17.2% and 27.8%, p=0.004) and significantly lower numbers of cells in TE (47.4±15.3 and 57.2±17.7) and total cells (66.2±18.5 and 77.5±20.7) compared to the control group (p=0.001).

Conclusions: Dietary quercetin supplementation has a detrimental effect on mouse embryo quality. Moreover, it did not show any beneficial effect on the ovary in both quantity and quality. This finding raises awareness of the general use of dietary quercetin supplements in infertile females.

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膳食槲皮素对小鼠卵母细胞提取和体外受精结果的影响

目的：研究槲皮素对小鼠卵母细胞和试管婴儿结果的影响：研究槲皮素对小鼠卵母细胞和试管婴儿结果的影响：雌性小鼠分为两组。给小鼠灌胃不含槲皮素的 0.2 mL 水（对照组）或含槲皮素 30 mg/kg 的 0.2 mL 水（槲皮素组），每天一次，连续 21 天。之后，对雌性小鼠进行体外受精。我们观察了槲皮素对取卵卵母细胞数、卵母细胞退化率、受精率、囊胚形成率和囊胚细胞数的影响：结果：与对照组相比，槲皮素组每只小鼠获得的卵母细胞数（27.3±6.7 和 27.2±5.8）、卵母细胞破碎率（28.4% 和 25.0%）、受精率（47.4% 和 50.6%）和囊胚形成率（34.8% 和 34.7%）均无差异。与对照组相比，槲皮素组孵化和孵出囊胚的比例明显降低（17.2% 和 27.8%，P=0.004），TE 细胞数（47.4±15.3 和 57.2±17.7）和总细胞数（66.2±18.5 和 77.5±20.7）也明显降低（P=0.001）：结论：补充槲皮素对小鼠胚胎质量有不利影响。结论：膳食补充槲皮素对小鼠胚胎质量有不利影响，而且对卵巢的数量和质量都没有益处。这一发现提高了人们对不孕女性普遍使用槲皮素膳食补充剂的认识。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Jornal Brasileiro de Reproducao Assistida OBSTETRICS & GYNECOLOGY-

CiteScore

3.30

自引率

6.70%

发文量