Integrating Genomic Data with the Development of CRISPR-Based Point-of-Care-Testing for Bacterial Infections.

IF 3.1 Q2 MICROBIOLOGY

Current Clinical Microbiology Reports Pub Date : 2024-01-01 Epub Date: 2024-10-15 DOI:10.1007/s40588-024-00236-7

Thanyapat Wanitchanon, Claire Chewapreecha, Chayasith Uttamapinant

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引用次数: 0

Abstract

Purpose of review: Bacterial infections and antibiotic resistance contribute to global mortality. Despite many infections being preventable and treatable, the lack of reliable and accessible diagnostic tools exacerbates these issues. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-based diagnostics has emerged as a promising solution. However, the development of CRISPR diagnostics has often occurred in isolation, with limited integration of genomic data to guide target selection. In this review, we explore the synergy between bacterial genomics and CRISPR-based point-of-care tests (POCT), highlighting how genomic insights can inform target selection and enhance diagnostic accuracy.

Recent findings: We review recent advances in CRISPR-based technologies, focusing on the critical role of target sequence selection in improving the sensitivity of CRISPR-based diagnostics. Additionally, we examine the implementation of these technologies in resource-limited settings across Asia and Africa, presenting successful case studies that demonstrate their potential.

Summary: The integration of bacterial genomics with CRISPR technology offers significant promise for the development of effective point-of-care diagnostics.

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将基因组数据与开发基于 CRISPR 的细菌感染护理点检测相结合。

审查目的：细菌感染和抗生素耐药性导致全球死亡率上升。尽管许多感染是可以预防和治疗的，但由于缺乏可靠和方便的诊断工具，这些问题变得更加严重。基于 CRISPR（Clustered Regularly Interspaced Short Palindromic Repeats）的诊断方法已成为一种前景广阔的解决方案。然而，CRISPR 诊断技术的开发往往是孤立进行的，对基因组数据的整合有限，无法指导目标选择。在这篇综述中，我们探讨了细菌基因组学与基于CRISPR的床旁检测（POCT）之间的协同作用，重点介绍了基因组学见解如何为目标选择提供信息并提高诊断准确性：我们回顾了基于 CRISPR 的技术的最新进展，重点关注目标序列选择在提高基于 CRISPR 的诊断灵敏度方面的关键作用。摘要：细菌基因组学与 CRISPR 技术的整合为开发有效的护理点诊断技术带来了巨大的希望。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Clinical Microbiology Reports MICROBIOLOGY-

CiteScore

7.50

自引率

1.90%

发文量

期刊介绍： Current Clinical Microbiology Reports commissions expert reviews from leading scientists at the forefront of research in microbiology. The journal covers this broad field by dividing it into four key main areas of study: virology, bacteriology, parasitology, and mycology. Within each of the four sections, experts from around the world address important aspects of clinical microbiology such as immunology, diagnostics, therapeutics, antibiotics and antibiotic resistance, and vaccines. Some of the world’s foremost authorities in the field of microbiology serve as section editors and editorial board members. Section editors select topics for which leading researchers are invited to contribute comprehensive review articles that emphasize new developments and recently published papers of major importance, which are highlighted in annotated reference lists. These timely reviews of the literature examine the latest scientific discoveries and controversies as they emerge and are indispensable to both researchers and clinicians. The editorial board, composed of more than 20 internationally diverse members, reviews the annual table of contents, ensures that topics address all aspects of emerging research, and where applicable suggests topics of critical importance to various countries/regions.