Evaluation of decellularized sheep kidney scaffolds for renal tissue engineering: Biocompatibility and stem cell differentiation potential

IF 2.7 4区生物学 Q1 ANATOMY & MORPHOLOGY

Tissue & cell Pub Date : 2024-11-08 DOI:10.1016/j.tice.2024.102594

Maryam Jahanvar , Saber Zahri , Arash Abdolmaleki , Asadollah Asadi

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引用次数: 0

Abstract

Tissue engineering (TE) combines scaffolds, cells, and bioactive chemicals in order to create tissues. The objective is to restore or sustain tissue functionality and expedite the recovery of damaged tissues or organs in a controlled laboratory environment. This study aimed to evaluate the properties and biocompatibility of decellularized sheep kidney scaffolds (DKS) and to explore the differentiation potential of adipose-derived mesenchymal stem cells (ADSCs) into renal cells. After decellularizing sheep kidneys using freeze-drying and detergent techniques, we conducted histological studies, DNA quantification, and ultrastructural evaluations using scanning electron microscopy (SEM). Furthermore, to assay the feasibility and attachment of stem cells to the decellularized scaffolds, ADSCs were cultured on the scaffolds and subjected to the MTT assay. The expression of the pax2 gene was analyzed using real-time PCR to determine the differentiation of MSCs into kidney cells. DNA quantitation revealed a significant reduction in the quantity of DNA present in the scaffold tissue compared to the control kidney tissue. Ultrastructural examination confirmed the preservation of the decellularized scaffold's ultrastructure. Histological analysis demonstrated the complete removal of nuclear material from the scaffold. Additionally, Pax2 gene expression was significantly increased in ADSC cells cultured on the scaffold compared to the control group. The results demonstrate that the produced scaffolds are well-suited for regenerative medicine, exhibiting excellent biocompatibility and providing a conducive environment for the differentiation of ADSCs.

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评估用于肾组织工程的脱细胞羊肾支架：生物相容性和干细胞分化潜力

组织工程（TE）将支架、细胞和生物活性化学物质结合起来，以制造组织。其目的是在可控的实验室环境中恢复或维持组织功能，加快受损组织或器官的恢复。本研究旨在评估脱细胞绵羊肾脏支架（DKS）的特性和生物相容性，并探索脂肪间充质干细胞（ADSCs）向肾脏细胞分化的潜力。使用冷冻干燥和去污剂技术对绵羊肾脏进行脱细胞处理后，我们进行了组织学研究、DNA定量以及使用扫描电子显微镜（SEM）进行超微结构评估。此外，为了检测干细胞在脱细胞支架上附着的可行性，我们在支架上培养了 ADSCs，并进行了 MTT 试验。实时PCR分析了pax2基因的表达，以确定间充质干细胞向肾细胞的分化。DNA 定量分析显示，与对照肾组织相比，支架组织中的 DNA 数量明显减少。超微结构检查证实脱细胞支架的超微结构得以保留。组织学分析表明，支架中的核物质被完全清除。此外，与对照组相比，在支架上培养的 ADSC 细胞的 Pax2 基因表达明显增加。这些结果表明，所制备的支架非常适合再生医学，具有良好的生物相容性，能为 ADSCs 的分化提供有利的环境。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Tissue & cell 医学-解剖学与形态学

CiteScore

3.90

自引率

0.00%

发文量

234

期刊介绍： Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed. Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.