Analysis of metabolites associated with ADIPOQ genotypes in individuals with type 2 diabetes mellitus.

IF 3.8 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Scientific Reports Pub Date : 2024-11-15 DOI:10.1038/s41598-024-79686-4

Tainá Gomes Diniz, Caroline Severo de Assis, Bruno Rafael Virginio de Sousa, Kamila Sabino Batista, Alexandre Sérgio Silva, Isabella Wanderley de Queiroga Evangelista, Marina Gonçalves Monteiro Viturino, Yuri Mangueira do Nascimento, Evandro Ferreira da Silva, Josean Fechine Tavares, Mussara Gomes Cavalcanti Alves Monteiro, Carla Patricia Novaes Dos Santos Fechine, Anauara Lima E Silva, Darlene Camati Persuhn

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Abstract

Diabetes mellitus (DM) is a significant public health problem and it is known that the identification of molecular markers involved in glycemic control can impact disease control. Although the rs266729 polymorphism located in the promoter of the adiponectin gene (ADP) has been shown to be a candidate for involvement in glycemic control, the genotypic groups have never been characterized in terms of metabolomic aspects. Objective: Analyze the metabolites present in the rs266729 genotype groups. 127 diabetic individuals were compared according to the rs266729 genotype groups CC and GC + GG (RFLP-PCR). Blood plasma metabolites were classified by nuclear magnetic resonance (NMR), and the metabolic pathways of each group using the MetaboAnalyst tool. Insulin therapy (p = 0.049) was more frequent in the GC + GG rs266729 group. Lactate, alanine, glutamine, aspartate, lipid, lysine, isoleucine, citrulline, cholesterol, and fucose impacted the CC group and aspartate, beta-glucose, glutamate, pyruvate, proline, and 2-oxoglutarate impacted the CG + GG group. The glucose-alanine pathway, malate-aspartate transport, and urea cycle impacted the CC group (D-glucose, glutamic acid, L-alanine, oxoglutaric acid, and pyruvic acid). The glutamine/glutamate ratio is likely to be related to the causes of rs266729 influencing the risk of diabetes.

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分析与 2 型糖尿病患者 ADIPOQ 基因型相关的代谢物。

糖尿病（DM）是一个重大的公共卫生问题，众所周知，确定参与血糖控制的分子标记物会影响疾病的控制。尽管位于脂肪连蛋白基因（ADP）启动子上的 rs266729 多态性已被证明是参与血糖控制的候选基因，但其基因型群体在代谢组学方面却从未被表征过。研究目的分析 rs266729 基因型群体中存在的代谢物。根据 rs266729 基因型组 CC 和 GC + GG（RFLP-PCR）对 127 名糖尿病患者进行比较。通过核磁共振（NMR）对血浆代谢物进行分类，并使用 MetaboAnalyst 工具对各组的代谢途径进行分类。GC + GG rs266729 组更常接受胰岛素治疗（p = 0.049）。乳酸、丙氨酸、谷氨酰胺、天门冬氨酸、脂质、赖氨酸、异亮氨酸、瓜氨酸、胆固醇和岩藻糖对 CC 组有影响，而天门冬氨酸、β-葡萄糖、谷氨酸、丙酮酸、脯氨酸和 2-氧代戊二酸对 CG + GG 组有影响。葡萄糖-丙氨酸途径、苹果酸-天门冬氨酸转运和尿素循环对 CC 组有影响（D-葡萄糖、谷氨酸、L-丙氨酸、氧谷氨酸和丙酮酸）。谷氨酰胺/谷氨酸比率可能与 rs266729 影响糖尿病风险的原因有关。

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来源期刊

Scientific Reports Natural Science Disciplines-

CiteScore

7.50

自引率

4.30%

发文量

19567

审稿时长

3.9 months

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