Establishment of acquired radioresistant cells to fractionated radiation from hTERT-immortalized normal human epithelial cell.

IF 0.8 4区环境科学与生态学 Q4 ENVIRONMENTAL SCIENCES

Radiation protection dosimetry Pub Date : 2024-11-13 DOI:10.1093/rpd/ncae118

Masatoshi Suzuki, Rio Isobe, Taku Sato, Ryoya Ishikawa, Keiji Suzuki, Yasushi Kino, Tomisato Miura, Yohei Inaba, Koichi Chida, Manabu Fukumoto

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引用次数: 0

Abstract

Senescence-like growth arrest (SLGA), which is a radiation-induced cell death pathway, is induced in immortalized normal human epithelial cell (hTERT-RPE1) by the daily fractionated X-irradiation with 1.5 Gy within 30 times. We here demonstrate that pre-treatment induces acquired radioresistance (ARR) that can survive from the lethal fractionated radiation. The parent cells were daily fractionated with 1.5 Gy for 5 d and then incubated for 7 d without fractionated radiation. After this, the daily fractionated radiation with 1.5 Gy was restarted. A small population of surviving cells appeared after 30 times of the daily fractionated radiation was completed and they were continuously growing up to 120 times of the daily fractionated radiation (RPE1-1.5Fr). We confirmed a higher basal expression level of p53, which functions in the activation of the SLGA pathway but fails to further accumulate after 1.5 Gy of single irradiation in RPE1-1.5Fr. It is the first report to induce ARR phenotype for fractionated radiation in normal human cells.

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从 hTERT-immortalized的正常人上皮细胞中建立获得性抗分化辐射细胞。

衰老样生长停滞（SLGA）是一种辐射诱导的细胞死亡途径，通过每天 30 次以内 1.5 Gy 的分次 X 射线照射，诱导永生的正常人上皮细胞（hTERT-RPE1）发生衰老样生长停滞。我们在此证明，预处理可诱导获得性放射抗性（ARR），使其在致命的分次辐射中存活下来。母细胞每天接受 1.5 Gy 分段辐射 5 天，然后在没有分段辐射的情况下培养 7 天。之后，重新开始每天 1.5 Gy 的分次辐射。在完成 30 次每日分次辐射后，出现了一小部分存活细胞，它们一直持续生长到 120 次每日分次辐射（RPE1-1.5Fr）。我们证实，在 RPE1-1.5Fr 中，p53 的基础表达水平较高，它在 SLGA 通路的激活过程中起作用，但在 1.5 Gy 单次照射后未能进一步积累。这是首次报道在正常人体细胞中诱导分次辐射的 ARR 表型。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Radiation protection dosimetry 环境科学-公共卫生、环境卫生与职业卫生

CiteScore

1.40

自引率

10.00%

发文量

223

审稿时长

6-12 weeks

期刊介绍： Radiation Protection Dosimetry covers all aspects of personal and environmental dosimetry and monitoring, for both ionising and non-ionising radiations. This includes biological aspects, physical concepts, biophysical dosimetry, external and internal personal dosimetry and monitoring, environmental and workplace monitoring, accident dosimetry, and dosimetry related to the protection of patients. Particular emphasis is placed on papers covering the fundamentals of dosimetry; units, radiation quantities and conversion factors. Papers covering archaeological dating are included only if the fundamental measurement method or technique, such as thermoluminescence, has direct application to personal dosimetry measurements. Papers covering the dosimetric aspects of radon or other naturally occurring radioactive materials and low level radiation are included. Animal experiments and ecological sample measurements are not included unless there is a significant relevant content reason.