MYB-1 regulates anthocyanin biosynthesis in Magnolia wufengensis

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2024-11-09 DOI:10.1016/j.plaphy.2024.109283

Xian-ping Liu , Min Luo , Xiu-qi Liu , Liu-yan Hao , Chen Zhu , Li Wang , Lv-yi Ma

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引用次数: 0

Abstract

Anthocyanin is an essential pigment in all major horticultural crops especially in ornamental trees. Magnolia wufengensis (new species of Magnolia) with red color flower was recently found as a popular species for ornamental use, but anthocyanin synthesis and regulation in M. wufengensis are poorly understood. Herein, transcriptome analysis was used to decipher the gene network associated with anthocyanin biosynthesis. An R2R3-like MwMYB-1 transcription factor was found. MwMYB-1 overexpression resulted in anthocyanin accumulation in tobacco and Arabidopsis. MwMYB-1 worked independently rather than forming a protein complex with bHLH or WD40 protein. According to MwMYB-1 DAP-seq analysis in Arabidopsis, the MwMYB-1 transcription factor preferred to bind the “AAGAGAG” motif (DREME-5) in the third exon of the AtMYB75 gene. The yeast one hybrid assay and transcription activity assay further confirmed this. Thus, MwMYB-1 activated AtMYB75 gene expression and conducted cascade amplification of anthocyanin biosynthesis. Taken together, our findings provide a novel understanding of anthocyanin biosynthesis regulation in M. wufengensis and can be used to promote agronomic trait improvement in tree species.

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MYB-1 调节五峰木兰的花青素生物合成。

花青素是所有主要园艺作物尤其是观赏树木中不可或缺的色素。花色为红色的五凤木兰（木兰属新种）是最近发现的一种常用观赏树种，但人们对五凤木兰中花青素的合成和调控知之甚少。本文利用转录组分析破译了与花青素生物合成相关的基因网络。研究发现了一种类似 R2R3 的 MwMYB-1 转录因子。MwMYB-1的过表达导致烟草和拟南芥中花青素的积累。MwMYB-1 独立工作，而不是与 bHLH 或 WD40 蛋白形成蛋白复合物。根据拟南芥中的 MwMYB-1 DAP-seq 分析，MwMYB-1 转录因子倾向于结合 AtMYB75 基因第三外显子中的 "AAGAGAG "基序（DREME-5）。酵母一杂交试验和转录活性试验进一步证实了这一点。因此，MwMYB-1 激活了 AtMYB75 基因的表达，并对花青素的生物合成进行了级联放大。综上所述，我们的研究结果提供了对五倍子花青素生物合成调控的新认识，可用于促进树种农艺性状的改良。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.