Multiomics analysis reveals the involvement of OnDIVARICATA 3 in controlling dynamic flower coloring of Oncidium hybridum

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2024-11-07 DOI:10.1016/j.plaphy.2024.109277

Yuanhua Luo , Yan Chen , Nengyan Fang, Lan Kong, Rongyan Lin, Yiquan Chen, Ronghui Fan, Huaiqin Zhong, Minling Huang, Xiuxian Ye

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Abstract

Flower color is one of the main quality and economic traits of ornamental plants, and a large amount of research on flower color mainly focuses on the differences between varieties, while there were few reports on the change of flower color at different developmental stages. In this study, the metabolome and transcriptome of a new strain ‘XM-1’ with dynamic color changes of Oncidium were analyzed. The results showed that rutin, quercetin and carotenoids metabolism decreased significantly during the change of color from yellow to white. Analyzing the correlation network between metabolites and differential expressed genes, 25 key structural genes were detected and regulated by multiple MYB-related transcription factors. The MYB-related transcription factor Cluster-100966.1_OnDIVARICATA 3 was selected for further analysis. The phylogenetic tree of DIVARICATA in different species of Orchidaceae and Arabidopsis thaliana was constructed and the most closely related members were selected for sequence comparison. The results showed that OnDIVARICATA 3 contained MYB-like conserved domains. Subcellular localization results showed that OnDIVARICATA 3 was located in the nucleus. In overexpressing OnDIVARICATA 3 transgenic hairy roots, the expression of flower color related genes FLS, ZEP, and CHYB were significantly up-regulated. In summary, this study characterized the key metabolic pathways in the formation of the dynamic flower color of Oncidium hybridum, and constructed the regulatory network of the MYB-related. These results laid a theoretical foundation for the subsequent research on flower color and genetic engineering technology breeding of Oncidium hybridum.

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多组学分析揭示了 OnDIVARICATA 3 参与控制 Oncidium hybridum 的动态花色。

花色是观赏植物的主要品质和经济性状之一，大量关于花色的研究主要集中在品种间的差异上，而关于不同发育阶段花色变化的报道较少。本研究分析了具有动态花色变化的新菌株 "XM-1 "的代谢组和转录组。结果表明，芦丁、槲皮素和类胡萝卜素的代谢在花色由黄色变为白色的过程中显著下降。通过分析代谢物与差异表达基因之间的相关网络，发现25个关键结构基因受多个MYB相关转录因子调控。选择 MYB 相关转录因子 Cluster-100966.1_OnDIVARICATA 3 作进一步分析。构建了兰科不同物种和拟南芥中 DIVARICATA 的系统发生树，并选择关系最密切的成员进行序列比较。结果表明，OnDIVARICATA 3含有类似MYB的保守结构域。亚细胞定位结果显示，OnDIVARICATA 3 位于细胞核中。在过表达 OnDIVARICATA 3 的转基因毛根中，花色相关基因 FLS、ZEP 和 CHYB 的表达显著上调。综上所述，本研究表征了杂交大花蕙兰动态花色形成的关键代谢途径，并构建了 MYB 相关的调控网络。这些结果为杂交鬼针草后续的花色研究和基因工程技术育种奠定了理论基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.