Mechanism of foliar application of boron to alleviate cadmium toxicity in winter wheat (Triticum aestivum L.)

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2024-11-01 DOI:10.1016/j.plaphy.2024.109264

Mengchan Xie, Yifan Feng, Peng Zhao, Zhaojun Nie, Hongen Liu, Wei Gao, Chang Li, Fuqing Sui, Long Wang, Shiyu Qin (秦世玉)

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引用次数: 0

Abstract

Our previous work shown that boron (B) can inhibit cadmium (Cd) absorption in winter wheat, while the mechanism is not well understood. In this study, the mechanism of foliage spray B on Cd uptake in Cd-contaminated farmland was characterized with three treatments: spraying distilled water (CK), spraying 0.3% B fertilizer (F0.3%), and spraying 0.6% B fertilizer (F0.6%). The spraying treatment was conducted during the jointing stage and booting stage, respectively. The objective was to investigate the impact of different concentrations of B fertilizer application on Cd content, yield, physiological biochemical response, Cd transporter in wheat. Results showed that the yield increased with increasing B spray concentration compared with CK treatment. The wheat yield increased by 1.29%∼12.50% under foliar B application. The Cd concentration in both shoot and root of wheat seedlings were significantly decreased with foliar application of B at jointing stage and booting stage. The Cd concentrations in root and seed were also decreased with B treatment at maturity stage, especially after treatment with F0.6%, the Cd absorption in wheat grains exhibited a significant reduction of 31.20%. In addition, foliar application of B significantly increased antioxidant enzyme activities, include APX, SOD, CAT, and POD. Compared with CK, the MDA content in leaves showed a significant decrease with 30.48%–50.14%, while the GSH content showed an increase with 76.32%–1.05%. The down-regulation of the uptake and transport genes (TaNramp5, TaLCT1 and TaHMA2) and the up-regulation of compartmentalization transport genes (TaTM20 and TaHMA3) may contribute to the reduction of Cd accumulation in shoot. Overall, our results suggest that foliar spraying of B could increase B accumulation and yield, and alleviate Cd toxicity by reducing Cd uptake, enhancing the antioxidant capacity, regulating the expression of Cd genes in wheat.

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叶面喷施硼缓解冬小麦（Triticum aestivum L.）镉毒性的机理。

我们之前的研究表明，硼（B）可以抑制冬小麦对镉（Cd）的吸收，但其机制尚不清楚。本研究采用三种处理，即喷洒蒸馏水（CK）、喷洒 0.3% 的硼肥（F0.3%）和喷洒 0.6% 的硼肥（F0.6%），研究了叶面喷施硼肥对镉污染农田中镉吸收的机理。喷洒处理分别在接穗期和出苗期进行。目的是研究施用不同浓度硼肥对小麦镉含量、产量、生理生化反应和镉转运体的影响。结果表明，与 CK 处理相比，随着硼肥喷施浓度的增加，产量也随之增加。叶面喷施硼肥的小麦产量增加了 1.29%∼12.50%。在拔节期和出苗期叶面喷施硼后，小麦幼苗的芽和根中的镉浓度都明显降低。成熟期叶面喷施硼处理后，小麦根部和种子中的镉浓度也有所下降，尤其是 F0.6% 叶面喷施硼处理后，小麦籽粒中的镉吸收量明显下降了 31.20%。此外，叶面喷施硼能显著提高抗氧化酶活性，包括 APX、SOD、CAT 和 POD。与 CK 相比，叶片中的 MDA 含量明显下降了 30.48%-50.14%，而 GSH 含量则增加了 76.32%-1.05%。吸收和转运基因（TaNramp5、TaLCT1 和 TaHMA2）的下调和分区转运基因（TaTM20 和 TaHMA3）的上调可能有助于减少镉在芽中的积累。总之，我们的研究结果表明，叶面喷施硼可以增加硼的积累和产量，并通过减少小麦对镉的吸收、提高抗氧化能力、调节镉基因的表达来减轻镉的毒性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.