Investigating the synergistic effects of nano-zinc and biochar in mitigating aluminum toxicity in soybeans

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2024-11-07 DOI:10.1016/j.plaphy.2024.109275

Enxi Zhang , Kun Liu , Suwen Liang , Lingrui Liu , Hai Nian , Tengxiang Lian

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Abstract

Aluminum (Al) toxicity limited root growth by reducing nutrient translocation and promoting reactive oxygen species (ROS) accumulation, particularly in soybean. The endophyte of root could be modified by plant metabolites, which could potentially alter the tolerance to environmental toxicity of plants in acidic-Al soils. To explore how they help soybean mitigate Al toxicity by altering root endophytes, zinc oxide nanoparticles (ZnO NPs) at doses of 0, 30, 60, 90 mg/kg and 2% biochar (BC) were selected as bio modifiers, and Al₂(SO₄)₃ at 19 mg/kg was used to simulate Al toxicity. We analyzed root endophytes and metabolites by high-throughput sequencing and gas chromatography-mass spectrometry (GC-MS). We found that ZnO NPs with BC could bolster soybean resilience against Al toxicity by enriching soil nutrients, activating enzymes, and bolstering antioxidant mechanisms. We also observed that it enriched root endophytic microbial diversity, notably increasing populations of Nakamurella, Aureimonas, Luteimonas, and Sphingomonas. These changes in the endophytes contributed to the improved adaptability of plants to adversity under Al toxicity. This study highlighted the potential of using ZnO NPs and BC as a sustainable approach to combat Al toxicity, emphasizing the intricate interplay between plant physiology and rhizosphere microbial dynamics in mitigating the effects of environmental toxicity.

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研究纳米锌和生物炭在减轻大豆铝毒性方面的协同效应。

铝（Al）毒性通过减少养分转运和促进活性氧（ROS）积累限制根系生长，尤其是在大豆中。根部的内生菌可被植物代谢产物改变，这有可能改变酸性铝土壤中植物对环境毒性的耐受性。为了探索如何通过改变根部内生菌来帮助大豆减轻铝毒性，我们选择了剂量为 0、30、60、90 毫克/千克的氧化锌纳米颗粒（ZnO NPs）和 2% 的生物炭（BC）作为生物调节剂，并使用 19 毫克/千克的 Al2(SO4)3 来模拟铝毒性。我们通过高通量测序和气相色谱-质谱（GC-MS）分析了根系内生菌和代谢物。我们发现，含有 BC 的 ZnO NPs 可通过富集土壤养分、激活酶和加强抗氧化机制来增强大豆对铝毒性的抗性。我们还观察到，它丰富了根部内生微生物的多样性，特别是增加了 Nakamurella、Aureimonas、Luteimonas 和 Sphingomonas 的数量。内生微生物的这些变化有助于提高植物对铝毒性逆境的适应能力。这项研究强调了使用氧化锌氮氧化物和碱性生物碱作为一种可持续方法来对抗铝毒性的潜力，同时强调了植物生理机能和根圈微生物动态之间在减轻环境毒性影响方面错综复杂的相互作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.