Pseudomonas putida triggers phosphorus bioavailability and P-transporters under different phosphate regimes to enhance maize growth

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2024-11-07 DOI:10.1016/j.plaphy.2024.109279

Tanya Singh , Nikita Bisht , Mohd Mogees Ansari , Puneet Singh Chauhan

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引用次数: 0

Abstract

The decline of available phosphorus in soil due to anthropogenic activities necessitates utilizing soil microorganisms that influence soil phosphorus levels. However, the specific mechanisms governing their interaction in Zea mays under diverse phosphate regimes remain largely unknown. The present study investigated the dynamics of phosphorus solubilization and the impact of organic acid supplementation in combination with the beneficial rhizobacterium Pseudomonas putida (RA) on maize growth under phosphorus-limiting and unavailable conditions. HPLC analysis revealed gluconic acid as the primary organic acid (OA) produced by P. putida across all three conditions (P-sufficient, P-limiting, and P-unavailable), with the highest production occurring under P-limiting conditions. The study evaluates the effects of RA, OA, and OA + RA on plant growth parameters under P-limiting and insufficient conditions, revealing significant alterations in growth and biochemical parameters (P = 0.05) compared to their respective untreated controls. Additionally, plants treated with organic acids and bacterial inoculation show increased phosphorus concentrations in both roots and shoots. Gene expression analysis of key phosphorus transporter genes (PHT1, PHO1, PTF, PHF1) further supports the role of organic acids and bacterial inoculation in enhancing phosphorus uptake. In conclusion, our study affirms that the secretion of gluconic acid by RA and its plant growth-promoting properties boost phosphorus uptake and maize growth by increasing phosphorus availability and influencing the expression of phosphorus transport-related genes.

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假单胞菌（Pseudomonas putida）在不同磷酸盐制度下触发磷生物利用率和磷转运体，以促进玉米生长。

人为活动导致土壤中可利用的磷减少，因此有必要利用影响土壤磷含量的土壤微生物。然而，在不同的磷酸盐制度下，玉米中微生物相互作用的具体机制在很大程度上仍不为人所知。本研究调查了磷溶解的动态以及有机酸补充与有益根瘤菌假单胞菌（RA）相结合对限磷和缺磷条件下玉米生长的影响。高效液相色谱分析显示，葡萄糖酸是腐生假单胞菌在所有三种条件（磷充足、磷限制和磷不可得）下产生的主要有机酸（OA），其中在磷限制条件下产量最高。研究评估了 RA、OA 和 OA + RA 在 P 限制和 P 不足条件下对植物生长参数的影响，结果显示，与各自未处理的对照组相比，植物的生长和生化参数发生了显著变化（P = 0.05）。此外，经有机酸和细菌接种处理的植物根部和芽中的磷浓度都有所增加。关键磷转运基因（PHT1、PHO1、PTF、PHF1）的基因表达分析进一步证实了有机酸和细菌接种在提高磷吸收方面的作用。总之，我们的研究证实，RA 分泌的葡萄糖酸及其促进植物生长的特性通过增加磷的可用性和影响磷转运相关基因的表达，促进了磷的吸收和玉米的生长。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.