Seroprevalence and associated risk factors of bovine herpesvirus 1 in smallholder dairy farms in two districts of Gondar zones, North-West Ethiopia

Abstract

Bovine herpesvirus 1 (BoHV-1) is the infectious agent that causes infectious bovine rhinotracheitis (IBR), a disease affecting the reproductive and respiratory systems of cattle. Significant economic losses result from infectious bovine rhinotracheitis because of metritis, abortions, placenta retention, recurrent breeding, animal deaths, and losses from trade restrictions. Reports of the diseases have been made in southern, southwestern and in major cities that kept improved breed of dairy cows in Ethiopia with prevalence ranging from 28.5–67 %. However, there is insufficient information available regarding the frequency and spread of IBR in the northwest part of the nation. In northwest Ethiopia, a cross-sectional study was carried out to estimate seroprevalence of BoHV-1 and identify associated risk factors. Dairy farms and farm owners were chosen using a multistage cluster sampling technique, while dairy cattle were chosen using a simple random sample technique. Four hundred and thirty-one dairy cattle from 177 herds in the Debark and Lay-Armachiho districts in the North and Central Gondar zones, respectively, both in Northwestern Ethiopia, were selected to provide serum samples. Owners of dairy animals provided information via questionnaires. Using a competitive enzyme-linked immunosorbent assay (c-ELISA), anti-BoHV-1 antibodies were detected in serum samples. To identify risk factors, univariable and multivariable mixed effect logistic regression models were used. We calculated animal level and herd level seroprevalence of 72 % (95 % CI: 64.9–78.4 %) and 85.7 % (95 % CI: 79.8–90 %), respectively. Parity was associated with seroprevalence of BoHV-1; cows with higher parity had increased seroprevalence of BoHV-1. Bull mating [OR=3.13, (95 % CI: 1.74–5.64)] compared to AI and Debark district [OR=2.73 (95 % CI: 1.63–4.57)] compared to Lay-Armachiho district, were associated with seroprevalence of BoHV-1. The study had shown that BoHV-1 is circulating out of the major cities and also on dairy farms keeping local breeds of dairy cows in Gondar zones, North-West Ethiopia. This suggests need of attention in prevention and control of BoHV-1.