Transcriptomic responses of Solanum tuberosum cv. Pirol to arbuscular mycorrhiza and potato virus Y (PVY) infection.

IF 3.9 2区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Edyta Deja-Sikora, Marcin Gołębiewski, Katarzyna Hrynkiewicz
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引用次数: 0

Abstract

Arbuscular mycorrhizal fungi (AMF) serve as both plant symbionts and allies in resisting pathogens and environmental stresses. Mycorrhizal colonization of plant roots can influence the outcomes of plant-pathogen interactions by enhancing specific host defense mechanisms. The transcriptional responses induced by AMF in virus-infected plants remain largely unexplored. In the presented study, we employed a comprehensive transcriptomic approach and qPCR to investigate the molecular determinants underlying the interaction between AMF and potato virus Y (PVY) in Solanum tuberosum L. Our primary goal was to identify the symbiosis- and defense-related determinants activated in mycorrhizal potatoes facing PVY. Through a comparative analysis of mRNA transcriptomes in experimental treatments comprising healthy and PVY-infected potatoes colonized by two AMF species, Rhizophagus regularis or Funneliformis mosseae, we unveiled the overexpression of genes associated with mycorrhiza, including nutrient exchange, lipid transfer, and cell wall remodeling. Furthermore, we identified several differentially expressed genes upregulated in all mycorrhizal treatments that encoded pathogenesis-related proteins involved in plant immune responses, thus verifying the bioprotective role of AMF. We investigated the relationship between mycorrhiza levels and PVY levels in potato leaves and roots. We found accumulation of the virus in the leaves of mycorrhizal plants, but our studies additionally showed a reduced PVY content in potato roots colonized by AMF, which has not been previously demonstrated. Furthermore, we observed that a virus-dependent reduction in nutrient exchange could occur in mycorrhizal roots in the presence of PVY. These findings provide an insights into the interplay between virus and AMF.

Solanum tuberosum cv. Pirol 对丛生菌根和马铃薯病毒 Y (PVY) 感染的转录组反应。
丛枝菌根真菌(AMF)既是植物的共生体,也是抵抗病原体和环境压力的盟友。菌根在植物根部的定殖可以通过增强特定的宿主防御机制来影响植物与病原体相互作用的结果。在病毒感染的植物中,AMF 诱导的转录反应在很大程度上仍未得到探索。在本研究中,我们采用了一种全面的转录组学方法和 qPCR 来研究 Solanum tuberosum L 中 AMF 与马铃薯病毒 Y(PVY)相互作用的分子决定因素。通过比较分析健康马铃薯和受 PVY 感染的马铃薯在两种 AMF(Rhizophagus regularis 或 Funneliformis mosseae)定殖下的 mRNA 转录组,我们发现了与菌根相关的基因的过度表达,包括营养交换、脂质转移和细胞壁重塑。此外,我们还发现了几个在所有菌根处理中上调的差异表达基因,这些基因编码与植物免疫反应有关的致病蛋白,从而验证了 AMF 的生物保护作用。我们研究了马铃薯叶片和根中菌根水平与 PVY 水平之间的关系。我们发现病毒在菌根植物的叶片中积累,但我们的研究还显示,被 AMF 定殖的马铃薯根中的 PVY 含量降低了,而这是以前从未证明过的。此外,我们还观察到,在有 PVY 存在的情况下,菌根植物根部的养分交换会出现依赖性减少。这些发现使我们对病毒和 AMF 之间的相互作用有了更深入的了解。
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来源期刊
Plant Molecular Biology
Plant Molecular Biology 生物-生化与分子生物学
自引率
2.00%
发文量
95
审稿时长
1.4 months
期刊介绍: Plant Molecular Biology is an international journal dedicated to rapid publication of original research articles in all areas of plant biology.The Editorial Board welcomes full-length manuscripts that address important biological problems of broad interest, including research in comparative genomics, functional genomics, proteomics, bioinformatics, computational biology, biochemical and regulatory networks, and biotechnology. Because space in the journal is limited, however, preference is given to publication of results that provide significant new insights into biological problems and that advance the understanding of structure, function, mechanisms, or regulation. Authors must ensure that results are of high quality and that manuscripts are written for a broad plant science audience.
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