Bacterial-host adhesion dominated by collagen subtypes remodelled by osmotic pressure.

IF 7.8 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

npj Biofilms and Microbiomes Pub Date : 2024-11-12 DOI:10.1038/s41522-024-00600-x

Hongwei Xu, Yuting Feng, Yongtao Du, Yiming Han, Xiaocen Duan, Ying Jiang, Liya Su, Xiaozhi Liu, Siying Qin, Kangmin He, Jianyong Huang

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Abstract

Environmental osmolarity plays a crucial role in regulating the functions and behaviors of both host cells and pathogens. However, it remains unclear whether and how environmental osmotic stimuli modulate bacterial‒host interfacial adhesion. Using single-cell force spectroscopy, we revealed that the interfacial adhesion force depended nonlinearly on the osmotic prestimulation of host cells but not bacteria. Quantitatively, the adhesion force increased dramatically from 25.98 nN under isotonic conditions to 112.45 or 93.10 nN after the host cells were treated with the hypotonic or hypertonic solution. There was a strong correlation between the adhesion force and the number of host cells harboring adherent/internalized bacteria. We further revealed that enhanced overexpression levels of collagen XV and II were responsible for the increases in interfacial adhesion under hypotonic and hypertonic conditions, respectively. This work provides new opportunities for developing host-directed antibacterial strategies related to interfacial adhesion from a mechanobiological perspective.

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渗透压重塑的胶原亚型主导细菌-宿主粘附。

环境渗透压在调节宿主细胞和病原体的功能和行为方面起着至关重要的作用。然而，环境渗透刺激是否以及如何调节细菌-宿主界面粘附力仍不清楚。我们利用单细胞力谱仪发现，界面粘附力与宿主细胞的渗透预刺激呈非线性关系，但与细菌无关。从数量上看，宿主细胞经低渗或高渗溶液处理后，粘附力从等渗条件下的 25.98 nN 剧增至 112.45 或 93.10 nN。粘附力与宿主细胞中粘附/内化细菌的数量之间存在很强的相关性。我们进一步发现，在低渗和高渗条件下，胶原蛋白 XV 和 II 的过表达水平增强分别是界面粘附力增强的原因。这项工作为从机械生物学角度开发与界面粘附相关的宿主定向抗菌策略提供了新的机遇。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

npj Biofilms and Microbiomes Immunology and Microbiology-Microbiology

CiteScore

12.10

自引率

3.30%

发文量

审稿时长

9 weeks

期刊介绍： npj Biofilms and Microbiomes is a comprehensive platform that promotes research on biofilms and microbiomes across various scientific disciplines. The journal facilitates cross-disciplinary discussions to enhance our understanding of the biology, ecology, and communal functions of biofilms, populations, and communities. It also focuses on applications in the medical, environmental, and engineering domains. The scope of the journal encompasses all aspects of the field, ranging from cell-cell communication and single cell interactions to the microbiomes of humans, animals, plants, and natural and built environments. The journal also welcomes research on the virome, phageome, mycome, and fungome. It publishes both applied science and theoretical work. As an open access and interdisciplinary journal, its primary goal is to publish significant scientific advancements in microbial biofilms and microbiomes. The journal enables discussions that span multiple disciplines and contributes to our understanding of the social behavior of microbial biofilm populations and communities, and their impact on life, human health, and the environment.