Endang Suprihati, Heni Puspitasari, Elly Nur Indasari, Ponasari Galuh, Lucia Tri Suwanti, Mufasirin Mufasirin, Poedji Hastutiek, Boedi Setiawan
{"title":"Molecular detection of <i>Cryptosporidium</i> spp. among wild rats in Surabaya, East Java, Indonesia.","authors":"Endang Suprihati, Heni Puspitasari, Elly Nur Indasari, Ponasari Galuh, Lucia Tri Suwanti, Mufasirin Mufasirin, Poedji Hastutiek, Boedi Setiawan","doi":"10.5455/OVJ.2024.v14.i10.18","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Humans and animals who have an acute case of diarrhea can be infected with <i>Cryptosporidium</i> spp. Within the category of water-borne disease, it is a zoonotic disease. The zoonotic disease <i>Cryptosporidium</i> is among the several pathogens carried by wild rats (<i>Rattus</i> spp.). The risk of spreading this disease is rather significant in urban environments because rats are often close to people.</p><p><strong>Aim: </strong>This study aims to detect Cryptosporidium spp. infection in wild rats in Surabaya, East Java, Indonesia.</p><p><strong>Methods: </strong>Through necropsy, a total of 100 wild rats' intestines were sampled for feces. Microscopic observation of the presence of <i>Cryptosporidium</i> was carried out using the float test with a combination of Ziehl Neelsen (ZN) staining. Molecular detection of <i>Cryptosporidium</i> spp. positive results used the <i>Cryptosporidium</i> oocyst wall protein (COWP) gene with polimerase chain reaction method.</p><p><strong>Results: </strong>The results showed that 69 samples were positive for containing <i>Cryptosporidium</i> spp. oocysts and with ZN staining to confirm the diagnosis, the staining results showed <i>Cryptosporidium</i> spp. oocysts dark pink with a clear cavity inside with a percentage of 95.65% in <i>Rattus norvegicus</i> and 61.03% in <i>Rattus tanezumi</i>. In residential and densely populated environments the percentage of <i>Cryptosporidium</i> spp. amounted to 66.66% and in the market environment amounted to 74.19%. The percentage of <i>Cryptosporidium</i> spp. in the North Surabaya region was 42.85%, South Surabaya 100%, West Surabaya 37.5%, East Surabaya 81.39%, and Central Surabaya 65.38%. Molecular detection of <i>Cryptosporidium</i> spp. positive results were obtained using the COWP gene 550 bp.</p><p><strong>Conclusion: </strong>This study aims to detect Cryptosporidium spp. infection in wild rats in Surabaya, East Java, Indonesia. The high number of cases of cryptosporidiosis in wild rats has the potential to be a reservoir for the spread of the disease. The Cryptosporidium spp can detected with COWP in 550 bp in wild rats in Surabaya, East Java, Indonesia.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":"14 10","pages":"2687-2692"},"PeriodicalIF":0.9000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11560268/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Open Veterinary Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5455/OVJ.2024.v14.i10.18","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/10/31 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Humans and animals who have an acute case of diarrhea can be infected with Cryptosporidium spp. Within the category of water-borne disease, it is a zoonotic disease. The zoonotic disease Cryptosporidium is among the several pathogens carried by wild rats (Rattus spp.). The risk of spreading this disease is rather significant in urban environments because rats are often close to people.
Aim: This study aims to detect Cryptosporidium spp. infection in wild rats in Surabaya, East Java, Indonesia.
Methods: Through necropsy, a total of 100 wild rats' intestines were sampled for feces. Microscopic observation of the presence of Cryptosporidium was carried out using the float test with a combination of Ziehl Neelsen (ZN) staining. Molecular detection of Cryptosporidium spp. positive results used the Cryptosporidium oocyst wall protein (COWP) gene with polimerase chain reaction method.
Results: The results showed that 69 samples were positive for containing Cryptosporidium spp. oocysts and with ZN staining to confirm the diagnosis, the staining results showed Cryptosporidium spp. oocysts dark pink with a clear cavity inside with a percentage of 95.65% in Rattus norvegicus and 61.03% in Rattus tanezumi. In residential and densely populated environments the percentage of Cryptosporidium spp. amounted to 66.66% and in the market environment amounted to 74.19%. The percentage of Cryptosporidium spp. in the North Surabaya region was 42.85%, South Surabaya 100%, West Surabaya 37.5%, East Surabaya 81.39%, and Central Surabaya 65.38%. Molecular detection of Cryptosporidium spp. positive results were obtained using the COWP gene 550 bp.
Conclusion: This study aims to detect Cryptosporidium spp. infection in wild rats in Surabaya, East Java, Indonesia. The high number of cases of cryptosporidiosis in wild rats has the potential to be a reservoir for the spread of the disease. The Cryptosporidium spp can detected with COWP in 550 bp in wild rats in Surabaya, East Java, Indonesia.
期刊介绍：
Open Veterinary Journal is a peer-reviewed international open access online and printed journal that publishes high-quality original research articles. reviews, short communications and case reports dedicated to all aspects of veterinary sciences and its related subjects. Research areas include the following: Infectious diseases of zoonotic/food-borne importance, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, epidemiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology, oncology and animal reproduction. All papers are peer-reviewed. Moreover, with the presence of well-qualified group of international referees, the process of publication will be done meticulously and to the highest standards.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
