Application of Human Plasma Targeted Lipidomics and Analysis of Toxic Elements to Capture the Metabolic Complexities of Hypothyroidism.

IF 4.2 2区化学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecules Pub Date : 2024-10-31 DOI:10.3390/molecules29215169

Anna Błażewicz, Michał Kiełbus, Katarzyna Skórzyńska-Dziduszko, Andreas M Grabrucker, Jacqueline Jonklaas, Piotr Sosnowski, Alicja Trzpil, Anna Kozub-Pędrak, Agnieszka Szmagara, Julia Wojnicka, Ewelina Grywalska, Agostinho Almeida

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Abstract

Background: Hypothyroidism (HT) affects millions worldwide and can lead to various lipid disorders. The metabolic complexity and the influence of toxic elements in autoimmune and non-autoimmune HT subtypes are not fully understood. This study aimed to investigate the relationships between plasma lipidome, toxic elements, and clinical classifications of HT in unexposed individuals.

Methods: Samples were collected from 120 adults assigned to a study group with Hashimoto's disease and non-autoimmune HT, and a healthy control group. Quantification of 145 pre-defined lipids was performed by using triple quadrupole tandem mass spectrometry (TQ MS/MS) in multiple reactions monitoring (MRM) mode via positive electrospray ionization (ESI). Levels of toxic elements were determined using inductively coupled plasma mass spectrometry (ICP-MS).

Results: Significant associations between altered levels of several components of the plasma lipidome and Al, Cd, Ni, As, and Pb with HT were found. We show metabolic differences in lysophosphatidylcholines (LPC) and phosphatidylcholines (PC) between HT and controls, with distinct predicted activation patterns for lysolecithin acyltransferase and phospholipase A2.

Conclusions: There are significant changes in the lipidome profiles of healthy subjects compared to euthyroid HT patients treated with L-thyroxine, which are related to the type of hypothyroidism and non-occupational exposure to toxic elements.

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应用人体血浆靶向脂质组学和有毒元素分析捕捉甲状腺机能减退的代谢复杂性。

背景：甲状腺功能减退症（HT）影响着全球数百万人，可导致各种脂质紊乱。目前还不完全清楚自身免疫性和非自身免疫性甲减亚型中代谢的复杂性和有毒元素的影响。本研究旨在调查未暴露个体血浆脂质体、有毒元素和 HT 临床分类之间的关系：收集了 120 名成年人的样本，他们被分配到桥本氏病和非自身免疫性 HT 研究组以及健康对照组。在多反应监测（MRM）模式下，通过正电喷雾离子化（ESI）使用三重四极杆串联质谱（TQ MS/MS）对 145 种预定脂质进行定量。采用电感耦合等离子体质谱法（ICP-MS）测定有毒元素的含量：结果：发现血浆脂质体的几种成分水平的改变与 HT 中的铝、镉、镍、砷和铅之间存在显著关联。我们显示了 HT 与对照组之间溶血磷脂酰胆碱（LPC）和磷脂酰胆碱（PC）的代谢差异，并预测了溶血磷脂酰基转移酶和磷脂酶 A2 的不同活化模式：结论：与接受左旋甲状腺素治疗的甲状腺功能正常的高密度脂蛋白血症患者相比，健康人的脂质体特征发生了明显变化，这与甲状腺功能减退症的类型和非职业接触有毒元素有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecules 化学-有机化学

CiteScore

7.40

自引率

8.70%

发文量

7524

审稿时长

1.4 months

期刊介绍： Molecules (ISSN 1420-3049, CODEN: MOLEFW) is an open access journal of synthetic organic chemistry and natural product chemistry. All articles are peer-reviewed and published continously upon acceptance. Molecules is published by MDPI, Basel, Switzerland. Our aim is to encourage chemists to publish as much as possible their experimental detail, particularly synthetic procedures and characterization information. There is no restriction on the length of the experimental section. In addition, availability of compound samples is published and considered as important information. Authors are encouraged to register or deposit their chemical samples through the non-profit international organization Molecular Diversity Preservation International (MDPI). Molecules has been launched in 1996 to preserve and exploit molecular diversity of both, chemical information and chemical substances.