Qiang Jin Mixture Promotes Osteogenic Differentiation of MC3T3-E1 Cells via BMP2/Smads Pathway and its Network Pharmacology Study.

IF 2.4 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Biotechnology Pub Date : 2024-11-11 DOI:10.1007/s12033-024-01313-4

Weiyue Gong, Yao Zhu, Limin Wang

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Abstract

The study aimed to explore the potential of QiangJin mixture (QJM), a Chinese herbal compound prescription, in regulating MC3T3-E1 cell differentiation and to analyze the ingredients and therapeutic targets of QJM against osteoporosis based on network pharmacology. MC3T3-E1 cells were incubated with different concentrations of QJM-contained rat serum (5, 10, or 20%). After 14 days of cell culture, Alizarin Red staining was performed to assess the mineralization ability of osteoblasts. RT-qPCR was used to measure mRNA levels of osteogenesis-related genes. Western blot was conducted to measure protein levels of factors related to the BMP2/Smads pathway. Functional and pathway enrichment of overlapping targets for QJM and osteoporosis were analyzed using gene ontology and KEGG analyses. As shown by experimental results, QJM-contained serum led to calcium deposition, increased expression levels of osteogenesis-related genes, and activated BMP2/Smad/Runx2 signaling in MC3T3-E1 cells. A total of 125 active compounds and 162 disease-related targets were identified. The core targets were MAPK8, TP53, ESR1, STAT3, MAPK3, IL6, NFKB1, JUN, MAPK1 and AKT1. In conclusion, QJM promotes the osteogenic differentiation of MC3T3-E1 cells by activating the BMP2/Smads signaling. Additionally, QJM is an anti-osteoporotic mixture by regulating diverse therapeutic targets and signaling pathways.

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羌金混剂通过 BMP2/Smads 通路促进 MC3T3-E1 细胞成骨分化及其网络药理学研究

本研究旨在探索中药复方羌活混合物（QJM）调节MC3T3-E1细胞分化的潜力，并基于网络药理学分析羌活混合物防治骨质疏松症的成分和治疗靶点。将 MC3T3-E1 细胞与不同浓度的含 QJM 的大鼠血清（5%、10% 或 20%）培养。细胞培养 14 天后，进行茜素红染色以评估成骨细胞的矿化能力。RT-qPCR 用于测量成骨相关基因的 mRNA 水平。采用 Western 印迹法测定 BMP2/Smads 通路相关因子的蛋白水平。利用基因本体论和 KEGG 分析方法分析了 QJM 和骨质疏松症重叠靶点的功能和通路富集。实验结果表明，含 QJM 的血清可导致 MC3T3-E1 细胞中钙的沉积、成骨相关基因表达水平的升高以及 BMP2/Smad/Runx2 信号的激活。共鉴定出 125 种活性化合物和 162 个疾病相关靶点。核心靶点包括 MAPK8、TP53、ESR1、STAT3、MAPK3、IL6、NFKB1、JUN、MAPK1 和 AKT1。总之，QJM 可通过激活 BMP2/Smads 信号促进 MC3T3-E1 细胞的成骨分化。此外，QJM 还能调节多种治疗靶点和信号通路，是一种抗骨质疏松的混合物。

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来源期刊

Molecular Biotechnology 医学-生化与分子生物学

CiteScore

4.10

自引率

3.80%

发文量

165

审稿时长

6 months

期刊介绍： Molecular Biotechnology publishes original research papers on the application of molecular biology to both basic and applied research in the field of biotechnology. Particular areas of interest include the following: stability and expression of cloned gene products, cell transformation, gene cloning systems and the production of recombinant proteins, protein purification and analysis, transgenic species, developmental biology, mutation analysis, the applications of DNA fingerprinting, RNA interference, and PCR technology, microarray technology, proteomics, mass spectrometry, bioinformatics, plant molecular biology, microbial genetics, gene probes and the diagnosis of disease, pharmaceutical and health care products, therapeutic agents, vaccines, gene targeting, gene therapy, stem cell technology and tissue engineering, antisense technology, protein engineering and enzyme technology, monoclonal antibodies, glycobiology and glycomics, and agricultural biotechnology.