Evaluations of a Cutaneous Wound Healing Model Using Oxygen Enhanced - Dynamic Contrast Enhanced Photoacoustic Imaging (OE-DCE PAI).

IF 3 4区医学 Q2 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING

Molecular Imaging and Biology Pub Date : 2024-11-12 DOI:10.1007/s11307-024-01966-2

Euitaek Yang, Alia Khaled, Xiaofei Liang, Jorge de la Cerda, F William Schuler, Shreya Goel, Mark D Pagel

{"title":"Evaluations of a Cutaneous Wound Healing Model Using Oxygen Enhanced - Dynamic Contrast Enhanced Photoacoustic Imaging (OE-DCE PAI).","authors":"Euitaek Yang, Alia Khaled, Xiaofei Liang, Jorge de la Cerda, F William Schuler, Shreya Goel, Mark D Pagel","doi":"10.1007/s11307-024-01966-2","DOIUrl":null,"url":null,"abstract":"Purpose: Preclinical models of cutaneous wound healing can be useful for improving clinical wound care. Oxygen Enhanced Photoacoustic imaging (OE PAI) can measure oxygenation, and Dynamic Contrast Enhanced (DCE) PAI can measure vascular perfusion. We investigated how a combined OE-DCE PAI protocol can measure vascular oxygenation and perfusion to a cutaneous healing model.Procedures: We developed a cutaneous \"punch\" wound model and photographed the wounds to track healing for 9 days. We performed OE-DCE PAI on Day 0, 3, 6, and 9. OE PAI was performed with 21% O2 and 100% O2 breathing gases to measure oxyhemoglobin (HbO2), deoxyhemoglobin (Hb), total hemoglobin (HbT), and oxygen saturation (%sO2), along with changes in these parameters caused by a change in breathing gas (ΔHb, ΔHbO2, ΔHbT, ΔsO2). To perform DCE PAI, indocyanine green (ICG) was administered intravenously while monitoring the PAI signal for 10 min as the agent washed through the wound area, which was used to evaluate the wash-out rate.Results: The average wound size was significantly smaller only by Day 6. For comparison, OE PAI measured a significant increase in HbO2, Hb, HbT, and %sO2 immediately after creating the wound, which significantly decreased by Day 3 and continued to decrease towards values for normal tissue by Day 9. The vascular wash-out rate significantly increased by Day 3, and continued to increase during the healing process. Notably, the wash-out rate can be assessed at a single PAI absorbance wavelength and by simply comparing signal amplitudes without advanced analysis, which may facilitate clinical translation.Conclusions: OE-DCE PAI can monitor significant changes in vascular perfusion and oxygenation prior to significant changes in cutaneous wound size. These results establish OE-DCE PAI as a tool for future pre-clinical wound healing studies and eventual clinical translation.","PeriodicalId":18760,"journal":{"name":"Molecular Imaging and Biology","volume":" ","pages":""},"PeriodicalIF":3.0000,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Imaging and Biology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s11307-024-01966-2","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING","Score":null,"Total":0}

引用次数: 0

Abstract

Purpose: Preclinical models of cutaneous wound healing can be useful for improving clinical wound care. Oxygen Enhanced Photoacoustic imaging (OE PAI) can measure oxygenation, and Dynamic Contrast Enhanced (DCE) PAI can measure vascular perfusion. We investigated how a combined OE-DCE PAI protocol can measure vascular oxygenation and perfusion to a cutaneous healing model.

Procedures: We developed a cutaneous "punch" wound model and photographed the wounds to track healing for 9 days. We performed OE-DCE PAI on Day 0, 3, 6, and 9. OE PAI was performed with 21% O₂ and 100% O₂ breathing gases to measure oxyhemoglobin (HbO₂), deoxyhemoglobin (Hb), total hemoglobin (HbT), and oxygen saturation (%sO₂), along with changes in these parameters caused by a change in breathing gas (ΔHb, ΔHbO₂, ΔHbT, ΔsO₂). To perform DCE PAI, indocyanine green (ICG) was administered intravenously while monitoring the PAI signal for 10 min as the agent washed through the wound area, which was used to evaluate the wash-out rate.

Results: The average wound size was significantly smaller only by Day 6. For comparison, OE PAI measured a significant increase in HbO₂, Hb, HbT, and %sO₂ immediately after creating the wound, which significantly decreased by Day 3 and continued to decrease towards values for normal tissue by Day 9. The vascular wash-out rate significantly increased by Day 3, and continued to increase during the healing process. Notably, the wash-out rate can be assessed at a single PAI absorbance wavelength and by simply comparing signal amplitudes without advanced analysis, which may facilitate clinical translation.

Conclusions: OE-DCE PAI can monitor significant changes in vascular perfusion and oxygenation prior to significant changes in cutaneous wound size. These results establish OE-DCE PAI as a tool for future pre-clinical wound healing studies and eventual clinical translation.

查看原文本刊更多论文

使用氧增强-动态对比度增强光声成像（OE-DCE PAI）评估皮肤伤口愈合模型。

目的：皮肤伤口愈合的临床前模型有助于改善临床伤口护理。氧增强光声成像（OE PAI）可以测量氧合，动态对比增强（DCE PAI）可以测量血管灌注。我们研究了 OE-DCE PAI 组合方案如何测量皮肤愈合模型的血管氧合和灌注：我们建立了一个皮肤 "打孔 "伤口模型，并对伤口进行拍照，以跟踪伤口 9 天的愈合情况。我们在第 0、3、6 和 9 天进行了 OE-DCE PAI。OE PAI 在 21%O2 和 100%O2 的呼吸气体中进行，测量氧合血红蛋白 (HbO2)、脱氧血红蛋白 (Hb)、总血红蛋白 (HbT) 和血氧饱和度 (%sO2)，以及呼吸气体变化引起的这些参数的变化（ΔHb、ΔHbO2、ΔHbT、ΔsO2）。在进行 DCE PAI 时，静脉注射吲哚菁绿（ICG），同时监测 PAI 信号 10 分钟，以评估洗脱率：结果：仅到第 6 天，平均伤口面积明显缩小。相比之下，OE PAI 在造成伤口后立即测得 HbO2、Hb、HbT 和 %sO2 显著增加，到第 3 天时显著减少，到第 9 天时继续向正常组织的值减少。到第 3 天，血管冲洗率明显增加，并在愈合过程中继续增加。值得注意的是，冲刷率可通过单一 PAI 吸收波长进行评估，只需比较信号振幅即可，无需进行高级分析，这可能有助于临床转化：结论：OE-DCE PAI 可以在皮肤伤口大小发生显著变化之前监测血管灌注和氧合的重大变化。这些结果确立了 OE-DCE PAI 作为未来临床前伤口愈合研究和最终临床转化的一种工具的地位。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Molecular Imaging and Biology 医学-核医学

CiteScore

6.90

自引率

3.20%

发文量

审稿时长

3 months

期刊介绍： Molecular Imaging and Biology (MIB) invites original contributions (research articles, review articles, commentaries, etc.) on the utilization of molecular imaging (i.e., nuclear imaging, optical imaging, autoradiography and pathology, MRI, MPI, ultrasound imaging, radiomics/genomics etc.) to investigate questions related to biology and health. The objective of MIB is to provide a forum to the discovery of molecular mechanisms of disease through the use of imaging techniques. We aim to investigate the biological nature of disease in patients and establish new molecular imaging diagnostic and therapy procedures. Some areas that are covered are: Preclinical and clinical imaging of macromolecular targets (e.g., genes, receptors, enzymes) involved in significant biological processes. The design, characterization, and study of new molecular imaging probes and contrast agents for the functional interrogation of macromolecular targets. Development and evaluation of imaging systems including instrumentation, image reconstruction algorithms, image analysis, and display. Development of molecular assay approaches leading to quantification of the biological information obtained in molecular imaging. Study of in vivo animal models of disease for the development of new molecular diagnostics and therapeutics. Extension of in vitro and in vivo discoveries using disease models, into well designed clinical research investigations. Clinical molecular imaging involving clinical investigations, clinical trials and medical management or cost-effectiveness studies.