Down-regulation of miR-138-5p in PP2A KO mice promoted apoptosis of spermatocytes.

IF 2.6 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Biology Reports Pub Date : 2024-11-13 DOI:10.1007/s11033-024-10096-5

Danni Wang, Xing Liu, Bingyan Chen, Yuwei Shang, Ting Wan, Shu Zhang, Huijun Liu, Yichao Shi, Xia Chen, Huiting Sun

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引用次数: 0

Abstract

Background: Protein phosphatase 2 A (PP2A) is known to have a pivotal and diverse functions in various physiological processes. In the previous study, we utilized the cre-loxp system to generate germ cell-specific knockout mice for the PP2A catalytic subunit alpha subunit (Ppp2ca^cKO).

Methods and results: Using high-throughput miRNA sequencing of testis tissues and real‑time PCR, we have identified a notable decrease in the expression of miR-138-5p in the testes of Ppp2ca^cKO mice. Our findings indicate that miR-138-5p plays a role in the regulation of apoptosis and proliferation of GC2 cells. Furthermore, bioinformatics analyses suggested that miR-138- 5p may target the transcriptional repressor Trps1. Consistent with these predictions, we observed a significant upregulation of Trps1 in the testes of Ppp2ca^cKO mice. Through transfection experiments, we have validated the negative regulation of Trps1 expression by miR-138-5p in GC2 cells.

Conclusion: Our study indicates that the down-regulation of miR-138-5p in PP2A KO mice, which targets Trps1 to promote spermatocyte apoptosis.

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PP2A KO 小鼠体内 miR-138-5p 的下调促进了精母细胞的凋亡。

背景：众所周知，蛋白磷酸酶2 A（PP2A）在各种生理过程中具有关键性的多种功能。在之前的研究中，我们利用 cre-loxp 系统生成了 PP2A 催化亚基 alpha 亚基特异性敲除小鼠（Ppp2cacKO）：通过对睾丸组织进行高通量 miRNA 测序和实时 PCR，我们发现 Ppp2cacKO 小鼠睾丸中 miR-138-5p 的表达明显减少。我们的研究结果表明，miR-138-5p 在调控 GC2 细胞的凋亡和增殖中发挥作用。此外，生物信息学分析表明，miR-138- 5p 可能以转录抑制因子 Trps1 为靶标。与这些预测一致，我们在 Ppp2cacKO 小鼠的睾丸中观察到 Trps1 的显著上调。通过转染实验，我们验证了 miR-138-5p 在 GC2 细胞中对 Trps1 表达的负调控作用：我们的研究表明，在 PP2A KO 小鼠体内，miR-138-5p 下调，从而靶向 Trps1 促进精母细胞凋亡。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Biology Reports 生物-生化与分子生物学

CiteScore

5.00

自引率

0.00%

发文量

1048

审稿时长

5.6 months

期刊介绍： Molecular Biology Reports publishes original research papers and review articles that demonstrate novel molecular and cellular findings in both eukaryotes (animals, plants, algae, funghi) and prokaryotes (bacteria and archaea).The journal publishes results of both fundamental and translational research as well as new techniques that advance experimental progress in the field and presents original research papers, short communications and (mini-) reviews.