Study on the molecular mechanism of atopic dermatitis in mice based on skin and serum metabolomic analysis.

IF 3.5 3区医学 Q2 ENDOCRINOLOGY & METABOLISM

Metabolomics Pub Date : 2024-11-13 DOI:10.1007/s11306-024-02196-x

Yingyue Wang, Xiaowei Chen, Chang Liu, Chunxue You, Yubin Xu

{"title":"Study on the molecular mechanism of atopic dermatitis in mice based on skin and serum metabolomic analysis.","authors":"Yingyue Wang, Xiaowei Chen, Chang Liu, Chunxue You, Yubin Xu","doi":"10.1007/s11306-024-02196-x","DOIUrl":null,"url":null,"abstract":"Introduction: Atopic dermatitis (AD) is a common chronic inflammatory dermatosis. However, the exact molecular mechanism underlying the development of AD remain largely unclear.Objective: To investigate comprehensive metabolomic alterations in serum and skin tissue between 2,4-dinitrofluorobenzene (DNFB)-induced AD-like mice and healthy controls, aiming to identify the potential disease biomarkers and explore the molecular mechanisms of AD.Methods: In this study, Untargeted metabolomics analysis was used to investigate both skin and serum metabolic abnormalities of 2,4-dinitrofluorobenzene (DNFB)-induced AD-like mice. Then, the metabolic differences among the groups were determined through the application of multivariate analysis. Additionally, the selection of predictive biomarkers was accomplished using the receiver operating characteristic (ROC) module.Results: Our findings showed that levels of 220 metabolites in the skin and 94 metabolites in the serum were different in AD-like mice that were treated with DNFB compared to control mice. Uracil, N-Acetyl-L-methionine, deoxyadenosine monophoosphate, 2-acetyl-l-alkyl-sn-glycero-3-phosphcholine, and prostaglandin D2 are considered potential biomarkers of AD as obtained by integrating skin and serum differential metabolite results. Metabolomic data analysis showed that the metabolic pathways in which skin and serum are involved together include histidine metabolism, pyrimidine metabolism, alanine, aspartate, and glutamate metabolism.Conclusion: Our research explained the possible molecular mechanism of AD at the metabolite level and provided potential targets for the development of clinical drugs for AD.","PeriodicalId":18506,"journal":{"name":"Metabolomics","volume":"20 6","pages":"131"},"PeriodicalIF":3.5000,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Metabolomics","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s11306-024-02196-x","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENDOCRINOLOGY & METABOLISM","Score":null,"Total":0}

引用次数: 0

Abstract

Introduction: Atopic dermatitis (AD) is a common chronic inflammatory dermatosis. However, the exact molecular mechanism underlying the development of AD remain largely unclear.

Objective: To investigate comprehensive metabolomic alterations in serum and skin tissue between 2,4-dinitrofluorobenzene (DNFB)-induced AD-like mice and healthy controls, aiming to identify the potential disease biomarkers and explore the molecular mechanisms of AD.

Methods: In this study, Untargeted metabolomics analysis was used to investigate both skin and serum metabolic abnormalities of 2,4-dinitrofluorobenzene (DNFB)-induced AD-like mice. Then, the metabolic differences among the groups were determined through the application of multivariate analysis. Additionally, the selection of predictive biomarkers was accomplished using the receiver operating characteristic (ROC) module.

Results: Our findings showed that levels of 220 metabolites in the skin and 94 metabolites in the serum were different in AD-like mice that were treated with DNFB compared to control mice. Uracil, N-Acetyl-L-methionine, deoxyadenosine monophoosphate, 2-acetyl-l-alkyl-sn-glycero-3-phosphcholine, and prostaglandin D2 are considered potential biomarkers of AD as obtained by integrating skin and serum differential metabolite results. Metabolomic data analysis showed that the metabolic pathways in which skin and serum are involved together include histidine metabolism, pyrimidine metabolism, alanine, aspartate, and glutamate metabolism.

Conclusion: Our research explained the possible molecular mechanism of AD at the metabolite level and provided potential targets for the development of clinical drugs for AD.

查看原文本刊更多论文

基于皮肤和血清代谢组学分析的小鼠特应性皮炎分子机制研究。

简介：特应性皮炎（AD）是一种常见的慢性炎症性皮肤病：特应性皮炎（AD）是一种常见的慢性炎症性皮肤病。然而，特应性皮炎发病的确切分子机制仍不清楚：研究2,4-二硝基氟苯（DNFB）诱导的类特应性皮炎小鼠与健康对照组之间血清和皮肤组织的全面代谢组学变化，旨在识别潜在的疾病生物标志物并探索类特应性皮炎的分子机制：本研究采用非靶向代谢组学分析方法研究了2,4-二硝基氟苯（DNFB）诱导的AD样小鼠的皮肤和血清代谢异常。然后，通过多变量分析确定了各组之间的代谢差异。此外，还利用接收器操作特征（ROC）模块完成了预测性生物标志物的选择：我们的研究结果表明，与对照组相比，接受 DNFB 治疗的 AD 样小鼠皮肤中 220 种代谢物和血清中 94 种代谢物的水平有所不同。综合皮肤和血清代谢物的差异结果，尿嘧啶、N-乙酰-L-蛋氨酸、脱氧腺苷单磷酸、2-乙酰基-l-烷基-sn-甘油-3-磷胆碱和前列腺素D2被认为是AD的潜在生物标志物。代谢组数据分析显示，皮肤和血清共同参与的代谢途径包括组氨酸代谢、嘧啶代谢、丙氨酸、天冬氨酸和谷氨酸代谢：我们的研究从代谢物水平解释了AD可能的分子机制，并为AD临床药物的开发提供了潜在靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Metabolomics 医学-内分泌学与代谢

CiteScore

6.60

自引率

2.80%

发文量

审稿时长

2 months

期刊介绍： Metabolomics publishes current research regarding the development of technology platforms for metabolomics. This includes, but is not limited to: metabolomic applications within man, including pre-clinical and clinical pharmacometabolomics for precision medicine metabolic profiling and fingerprinting metabolite target analysis metabolomic applications within animals, plants and microbes transcriptomics and proteomics in systems biology Metabolomics is an indispensable platform for researchers using new post-genomics approaches, to discover networks and interactions between metabolites, pharmaceuticals, SNPs, proteins and more. Its articles go beyond the genome and metabolome, by including original clinical study material together with big data from new emerging technologies.