Comprehensive gene expression analysis in gallbladder mucosal epithelial cells of dogs with gallbladder mucocele.

IF 2.6 2区农林科学

Journal of Veterinary Internal Medicine Pub Date : 2024-11-12 DOI:10.1111/jvim.17157

Itsuma Nagao, Tomoki Motegi, Yuko Goto-Koshino, Masaya Tsuboi, Naohiro Takahashi, James K Chambers, Kazuyuki Uchida, Kenji Baba, Hirotaka Tomiyasu, Masaru Okuda

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引用次数: 0

Abstract

Background: Gallbladder mucocele (GBM) is a common disease in the canine gallbladder. Although the pathogenesis of GBM remains unclear, we recently reported that the excessive accumulation of mucin in the gallbladder is not a result of overproduction by gallbladder epithelial cells (GBECs).

Hypothesis/objectives: Changes in the function of GBECs other than the production of mucin are associated with the pathogenesis of GBM. We performed an RNA sequencing (RNA-seq) analysis to comprehensively search for abnormalities in gene expression profiles of GBECs in dogs with GBM.

Animals: Fifteen dogs with GBM and 8 dogs euthanized for reasons other than gallbladder disease were included.

Methods: The GBECs were isolated from gallbladder tissues to extract RNA. The RNA-seq analysis was performed using the samples from 3 GBM cases and 3 dogs with normal gallbladders, and the gene expression profiles were compared between the 2 groups. Differences in mRNA expression levels of the extracted differentially expressed genes (DEGs) were validated by quantitative reverse transcription polymerase chain reaction (RT-qPCR) using samples of 15 GBM cases and 8 dogs with normal gallbladders.

Results: Comparison of gene expression profiles by RNA-seq extracted 367 DEGs, including ANO1, a chloride channel associated with changes in mucin morphology, and HTR4, which regulates the function of chloride channels. The ANO1 and HTR4 genes were confirmed to be downregulated in the GBM group by RT-qPCR.

Conclusions and clinical importance: Our results suggest that GBM may be associated with decreased function of chloride channels expressed in GBECs.

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对患有胆囊粘液瘤的狗的胆囊粘膜上皮细胞进行全面的基因表达分析。

背景：胆囊粘液瘤（GBM）是犬胆囊中的一种常见疾病。虽然 GBM 的发病机制仍不清楚，但我们最近报告说，胆囊中粘蛋白的过度积聚并不是胆囊上皮细胞（GBECs）过度分泌的结果：假设/目的：胆囊上皮细胞（GBECs）除产生粘蛋白外，其功能的变化与胆囊癌的发病机制有关。我们进行了一项 RNA 测序（RNA-seq）分析，以全面搜索 GBM 患犬 GBECs 基因表达谱的异常情况：动物：15 只患有 GBM 的狗和 8 只因胆囊疾病以外的原因安乐死的狗：从胆囊组织中分离 GBECs，提取 RNA。利用 3 只 GBM 病例和 3 只胆囊正常的狗的样本进行了 RNA-seq 分析，并比较了两组狗的基因表达谱。使用 15 例 GBM 病例和 8 例胆囊正常的狗的样本，通过定量反转录聚合酶链反应（RT-qPCR）验证了提取的差异表达基因（DEGs）mRNA 表达水平的差异：结果：通过 RNA-seq 对基因表达谱进行比较，提取出了 367 个 DEGs，其中包括与粘蛋白形态变化相关的氯离子通道 ANO1 和调节氯离子通道功能的 HTR4。RT-qPCR证实ANO1和HTR4基因在GBM组中下调：我们的研究结果表明，GBM 可能与 GBECs 中表达的氯离子通道功能下降有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Veterinary Internal Medicine Veterinary-General Veterinary

自引率

11.50%

发文量

243

期刊介绍： The mission of the Journal of Veterinary Internal Medicine is to advance veterinary medical knowledge and improve the lives of animals by publication of authoritative scientific articles of animal diseases.