Experimental arboviral infection of mosquito larvae: A novel approach for vector competence studies

IF 2.2 4区 医学 Q3 BIOCHEMICAL RESEARCH METHODS
Christin Körsten, Mandy Schäfer
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引用次数: 0

Abstract

Vector competence studies in mosquitoes present valuable opportunities to explore arboviral transmission and virus-vector interactions. However, oral infection studies in mosquitoes can be challenging. An alternative approach is to infect mosquitoes during their aquatic larval stage, resulting in the emergence of infected adults. To investigate the potential of this method, Culex pipiens biotype molestus larvae were infected with Usutu virus (USUV, Orthoflavivirus usutuense). For this purpose, larvae were exposed to USUV-infected mammalian and mosquito cell cultures for 24 h before being reared to adults. Subsequent analysis via RT-qPCR revealed that the Culex larvae successfully acquired USUV from the infected cells and exhibited high susceptibility to infection. Immediately after emergence, 32.10 % (26/81) of male and 41.03 % (16/39) of female mosquitoes tested positive for USUV RNA. Notably, females that were incubated for 15 days post-emergence demonstrated even higher infection rates, reaching 100.00 % (23/23). In addition, viral RNA and infectious particles were detected in some saliva samples, indicating the potential for transmission. This experimental infection of mosquito larvae thus offers the opportunity to produce infected adult mosquitoes for studies enhancing our understanding of virus-vector interactions, co-infections, and transmission routes. Such research contributes to better public health strategies addressing arboviral diseases.
蚊子幼虫的虫媒病毒实验感染:病媒能力研究的新方法。
蚊子的病媒能力研究为探索虫媒病毒传播和病毒与病媒的相互作用提供了宝贵的机会。然而,蚊子口腔感染研究具有挑战性。另一种方法是在蚊子的水生幼虫阶段对其进行感染,从而产生受感染的成虫。为了研究这种方法的潜力,我们用乌苏图病毒(USUV,Orthoflavivirus usutuense)感染了库蚊(Culex pipiens biotype molestus)幼虫。为此,在将幼虫饲养成成虫之前,先将其暴露于受 USUV 感染的哺乳动物和蚊子细胞培养物中 24 小时。随后的 RT-qPCR 分析表明,库蚊幼虫成功地从受感染的细胞中获得了 USUV,并表现出对感染的高度敏感性。刚出生时,32.10%(26/81)的雄蚊和 41.03%(16/39)的雌蚊的 USUV RNA 检测呈阳性。值得注意的是,出壳后孵化 15 天的雌蚊感染率更高,达到 100.00 %(23/23)。此外,在一些唾液样本中检测到了病毒 RNA 和传染性颗粒,这表明病毒有传播的可能性。因此,对蚊子幼虫的这种实验性感染为产生受感染的成蚊提供了机会,以进行研究,加深我们对病毒-媒介相互作用、合并感染和传播途径的了解。这些研究有助于制定更好的公共卫生策略来应对虫媒病毒疾病。
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来源期刊
CiteScore
5.80
自引率
0.00%
发文量
209
审稿时长
41 days
期刊介绍: The Journal of Virological Methods focuses on original, high quality research papers that describe novel and comprehensively tested methods which enhance human, animal, plant, bacterial or environmental virology and prions research and discovery. The methods may include, but not limited to, the study of: Viral components and morphology- Virus isolation, propagation and development of viral vectors- Viral pathogenesis, oncogenesis, vaccines and antivirals- Virus replication, host-pathogen interactions and responses- Virus transmission, prevention, control and treatment- Viral metagenomics and virome- Virus ecology, adaption and evolution- Applied virology such as nanotechnology- Viral diagnosis with novelty and comprehensive evaluation. We seek articles, systematic reviews, meta-analyses and laboratory protocols that include comprehensive technical details with statistical confirmations that provide validations against current best practice, international standards or quality assurance programs and which advance knowledge in virology leading to improved medical, veterinary or agricultural practices and management.
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