Comparison of effects of intra-articular diclofenac etalhyaluronate and hyaluronic acid in a monoiodoacetate rat osteoarthritis model.

IF 2.1 3区 医学 Q2 ORTHOPEDICS
Soichiro Tokeshi, Miyako Suzuki-Narita, Ikuko Tajiri, Kazuhide Inage, Jun Takeuchi, Takahito Arai, Yuya Kawarai, Hiroakira Terakawa, Seiji Ohtori, Sumihisa Orita
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引用次数: 0

Abstract

Diclofenac etalhyaluronate (DF-HA) sustained diclofenac release with the effects of hyaluronic acid (HA), offering long-term analgesia in osteoarthritis. In this study, the effects of DF-HA on pain improvement and osteoarthritis were evaluated in a rat knee monoiodoacetate-induced osteoarthritis model compared to HA. Eight rats per group had been injected with monoiodoacetate (2.0 mg) or saline in the right knee for 4 weeks and were injected with either DF-HA (1.25 mg/kg; 0.5 mg), HA (0.5 mg), vehicle which was a substrate without DF-HA (50 μL), or saline and followed for 4 weeks. Mechanical plantar skin sensitivity was assessed weekly using the von Frey assay. Osteoarthritis changes were monitored with Larsen scores via CT imaging at every 2 weeks. The articular cartilage was analyzed using OARSI scores through H&E, Safranin-O staining at 8 weeks. The percentage of Iba-1 positive microglia in the spinal dorsal horn and of FG + CGRP-labeled cells among FG-positive cells in the dorsal root ganglion were evaluated by immunohistochemical staining. TNF-α and IL-6 mRNA expression levels in the knee synovium were evaluated by PCR. The DF-HA showed significantly improved pain hypersensitivity compared with the HA at 6-8 weeks. The percentage of Iba-1-positive microglia was significantly lower than that in the vehicle and the percentage of FG + CGRP/FG was significantly lower than that in the HA. OARSI scores did not differ among treatment groups, Larsen scores indicated lower in the DF-HA than in the vehicle. DF-HA was as effective as HA in joint protection and significantly improved inflammatory pain compared to HA.

在单碘乙酸大鼠骨关节炎模型中比较关节内双氯芬酸乙醛酸盐和透明质酸的作用。
双氯芬酸乙醛uronate(DF-HA)可在透明质酸(HA)的作用下持续释放双氯芬酸,从而为骨关节炎提供长期镇痛效果。本研究在大鼠膝关节单碘乙酸盐诱导的骨关节炎模型中评估了 DF-HA 与 HA 相比对疼痛改善和骨关节炎的影响。每组 8 只大鼠右膝盖注射单碘乙酸盐(2.0 毫克)或生理盐水 4 周,然后分别注射 DF-HA(1.25 毫克/千克;0.5 毫克)、HA(0.5 毫克)、不含 DF-HA 的基质载体(50 μL)或生理盐水 4 周。每周使用 von Frey 试验评估机械性足底皮肤敏感性。每两周通过 CT 成像用 Larsen 评分监测骨关节炎的变化。8周时,通过H&E和Safranin-O染色,使用OARSI评分对关节软骨进行分析。免疫组化染色法评估了脊髓背角 Iba-1 阳性小胶质细胞的百分比和背根神经节 FG 阳性细胞中 FG + CGRP 标记细胞的百分比。通过 PCR 评估了膝关节滑膜中 TNF-α 和 IL-6 mRNA 的表达水平。6-8周后,DF-HA与HA相比,痛觉过敏性明显改善。Iba-1阳性小胶质细胞的比例明显低于药物,FG + CGRP/FG的比例明显低于HA。各治疗组的 OARSI 评分无差异,DF-HA 治疗组的 Larsen 评分低于药物治疗组。与 HA 相比,DF-HA 在保护关节方面与 HA 同样有效,并能明显改善炎症性疼痛。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Orthopaedic Research®
Journal of Orthopaedic Research® 医学-整形外科
CiteScore
6.10
自引率
3.60%
发文量
261
审稿时长
3-6 weeks
期刊介绍: The Journal of Orthopaedic Research is the forum for the rapid publication of high quality reports of new information on the full spectrum of orthopaedic research, including life sciences, engineering, translational, and clinical studies.
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