Akkermansia muciniphila Promotes SIgA Production and Alters the Reactivity Towards Commensal Bacteria in Early-Weaned Piglets.

IF 3.7 3区医学 Q2 NUTRITION & DIETETICS

Journal of Nutrition Pub Date : 2024-11-09 DOI:10.1016/j.tjnut.2024.11.002

Qin Jiang, Xiaoyan Zhu, Lingling Sun, Chunlin Xie, Xinkai Wang, Libao Ma, Xianghua Yan

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引用次数: 0

Abstract

Background: Secretory IgA (SIgA) is the first line of defense in protecting the intestinal epithelium against pathogenic bacteria, regulating gut microbiota composition, and maintaining intestinal homeostasis. Early weaning strategies may disrupt SIgA levels in piglet intestines, causing a decline in immune response and early weaning stress. However, the specific microbial mechanisms modulating SIgA in early-weaned piglets are not well understood.

Objective: We hypothesized that Akkermansia muciniphila increases intestinal SIgA production in the early-weaned piglets.

Methods: Fecal SIgA levels, SIgA-coated bacteria abundance, and fecal metagenomes were compared between 6 Huanjiang miniature (HM) and 6 Duroc×Landrace×Yorkshire (DLY) early-weaned piglets to identify bacterial species involved in SIgA modulation. 4 bacterial species were investigated using 5 groups (Control, A. muciniphila, L. amylovorus, L. crispatus, L. acidophilus) of male SPF C57BL/6J mice, weaned 3 weeks post-birth (n=8/group). Subsequently, 10-day-old Landrace×Yorkshire (LY) piglets were randomly assigned to three groups (Control, 10⁹A. muciniphila, 10⁸A. muciniphila) (n=10/group) to evaluate the effect of orally administered A. muciniphila on intestinal SIgA production and microbial composition.

Results: HM early-weaned piglets showed significantly higher SIgA levels (7.59 μg/mg, 95% CI: 3.2, 12, P = 0.002) and SIgA-coated bacteria abundance (8.64%, 95% CI: 3.2, 14, P = 0.014) than DLY piglets. In the mouse model, administration of A. muciniphila significantly increased SIgA levels (3.50 μg/mg, 95% CI: 0.59, 6.4, P = 0.018), SIgA-coated bacteria abundance (9.06%, 95% CI: 4, 14, P =0.018), and IgA⁺ plasma cell counts (6.1%, 95% CI: 4.3, 8, P = 0.005). In the pig experiments, oral administration of A. muciniphila to LY piglets significantly enhanced intestinal SIgA concentrations (4.22μg/mg, 95% CI: 0.37, 8.5, P = 0.034) and altered the SIgA-coated bacterial landscape.

Conclusion: Early intervention with A. muciniphila in nursing piglets can increases intestinal SIgA productionand alter the reactivity towards commensal bacteria upon early weaning.

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Akkermansia muciniphila 促进早期断奶仔猪 SIgA 的产生并改变其对共生细菌的反应性

背景：分泌型 IgA（SIgA）是保护肠道上皮免受病原菌侵害、调节肠道微生物群组成和维持肠道平衡的第一道防线。早期断奶策略可能会破坏仔猪肠道中的 SIgA 水平，导致免疫反应下降和早期断奶应激。然而，调节早期断奶仔猪 SIgA 的具体微生物机制尚不十分清楚：我们假设 Akkermansia muciniphila 能增加早期断奶仔猪肠道 SIgA 的产生：方法：比较了 6 头环江小型猪（HM）和 6 头杜洛克×兰氏×约克夏（DLY）早期断奶仔猪的粪便 SIgA 水平、SIgA 包被菌丰度和粪便元基因组，以确定参与 SIgA 调节的细菌种类。使用出生后 3 周断奶的雄性 SPF C57BL/6J 小鼠的 5 个组（对照组、粘液淀粉样小鼠、淀粉样小鼠、L. crispatus 小鼠、L. acidophilus 小鼠）对 4 种细菌进行了研究（n=8/组）。随后，将 10 日龄的兰德瑞斯×约克夏（LY）仔猪随机分为三组（对照组、109A. muciniphila 组、108A. muciniphila 组）（n=10/组），以评估口服 A. muciniphila 对肠道 SIgA 产生和微生物组成的影响：结果：HM 早期断奶仔猪的 SIgA 水平（7.59 μg/mg，95% CI：3.2，12，P = 0.002）和 SIgA 包被菌丰度（8.64%，95% CI：3.2，14，P = 0.014）明显高于 DLY 仔猪。在小鼠模型中，给药 A. muciniphila 可显著提高 SIgA 水平（3.50 μg/mg，95% CI：0.59，6.4，P = 0.018）、SIgA 包被细菌数量（9.06%，95% CI：4，14，P =0.018）和 IgA+ 浆细胞计数（6.1%，95% CI：4.3，8，P = 0.005）。在猪实验中，给 LY 仔猪口服 A. muciniphila 可显著提高肠道 SIgA 浓度（4.22μg/mg，95% CI：0.37, 8.5，P = 0.034），并改变 SIgA 包裹的细菌景观：结论：对哺乳仔猪使用粘多糖进行早期干预可增加肠道 SIgA 的分泌，并改变早期断奶仔猪对共生细菌的反应性。

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来源期刊

Journal of Nutrition 医学-营养学

CiteScore

7.60

自引率

4.80%

发文量

260

审稿时长

39 days

期刊介绍： The Journal of Nutrition (JN/J Nutr) publishes peer-reviewed original research papers covering all aspects of experimental nutrition in humans and other animal species; special articles such as reviews and biographies of prominent nutrition scientists; and issues, opinions, and commentaries on controversial issues in nutrition. Supplements are frequently published to provide extended discussion of topics of special interest.