{"title":"Imaging and observation of microcirculation in bowel mucosa using sidestream dark field imaging.","authors":"Keming Jiang, Lihong Chen, Hengyu Zhao, Huanxin Hu, Sicong Lai, Xinzhe Zhao, Hongda Zhang, Jia Ke, Qiongyu Hu","doi":"10.1111/jmi.13367","DOIUrl":null,"url":null,"abstract":"<p><p>Sidestream dark field (SDF) imaging is a tool for assessing microcirculation, commonly used for early diagnosis and monitoring of sepsis. In this study, we used SDF imaging to observe and assess the microcirculation of the intestinal mucosa during bowel surgery. We also compared different performance between normal mucosa and diseased mucosa using SDF imaging. SDF imaging was conducted in 13 patients to evaluate microcirculation parameters. All patients were assessed at distances of 0, 1, 2, 3 and 4 centimeters (cm) from the edge of the mesentery, respectively. Microcirculatory parameters such as microvascular flow index (MFI), proportion of perfused vessels (PPV), vascular density (VD), total vessel density (TVD), perfused vessel density (PVD) and heterogeneity index (HI) were measured in these patients. Compared to normal intestinal mucosa, the diseased intestinal mucosa exhibited higher values for VD (p = 0.044), TVD (p = 0.006) and PVD (p = 0.007). No significant differences in PPV, MFI and HI were observed between the two groups. The microcirculation parameters (MFI, PPV and PVD) of the intestine at the distal distance of 3 cm were significantly lower than those at a distance of 2 cm (MFI 1.5 (0.75) vs. 3 (0.5), PPV 57.6 (9.1) vs. 97.1 (8.6)% and PVD 11.395 (3.082) vs. 20.726 (4.115) mm/mm<sup>2</sup>). In conclusion, SDF imaging is an advanced technique that provide real-time visualization of intestinal mucosal microcirculation. It has the potential to assess the blood perfusion of the intestine during surgery.</p>","PeriodicalId":16484,"journal":{"name":"Journal of microscopy","volume":" ","pages":""},"PeriodicalIF":1.5000,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of microscopy","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1111/jmi.13367","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROSCOPY","Score":null,"Total":0}
引用次数: 0
Abstract
Sidestream dark field (SDF) imaging is a tool for assessing microcirculation, commonly used for early diagnosis and monitoring of sepsis. In this study, we used SDF imaging to observe and assess the microcirculation of the intestinal mucosa during bowel surgery. We also compared different performance between normal mucosa and diseased mucosa using SDF imaging. SDF imaging was conducted in 13 patients to evaluate microcirculation parameters. All patients were assessed at distances of 0, 1, 2, 3 and 4 centimeters (cm) from the edge of the mesentery, respectively. Microcirculatory parameters such as microvascular flow index (MFI), proportion of perfused vessels (PPV), vascular density (VD), total vessel density (TVD), perfused vessel density (PVD) and heterogeneity index (HI) were measured in these patients. Compared to normal intestinal mucosa, the diseased intestinal mucosa exhibited higher values for VD (p = 0.044), TVD (p = 0.006) and PVD (p = 0.007). No significant differences in PPV, MFI and HI were observed between the two groups. The microcirculation parameters (MFI, PPV and PVD) of the intestine at the distal distance of 3 cm were significantly lower than those at a distance of 2 cm (MFI 1.5 (0.75) vs. 3 (0.5), PPV 57.6 (9.1) vs. 97.1 (8.6)% and PVD 11.395 (3.082) vs. 20.726 (4.115) mm/mm2). In conclusion, SDF imaging is an advanced technique that provide real-time visualization of intestinal mucosal microcirculation. It has the potential to assess the blood perfusion of the intestine during surgery.
期刊介绍:
The Journal of Microscopy is the oldest journal dedicated to the science of microscopy and the only peer-reviewed publication of the Royal Microscopical Society. It publishes papers that report on the very latest developments in microscopy such as advances in microscopy techniques or novel areas of application. The Journal does not seek to publish routine applications of microscopy or specimen preparation even though the submission may otherwise have a high scientific merit.
The scope covers research in the physical and biological sciences and covers imaging methods using light, electrons, X-rays and other radiations as well as atomic force and near field techniques. Interdisciplinary research is welcome. Papers pertaining to microscopy are also welcomed on optical theory, spectroscopy, novel specimen preparation and manipulation methods and image recording, processing and analysis including dynamic analysis of living specimens.
Publication types include full papers, hot topic fast tracked communications and review articles. Authors considering submitting a review article should contact the editorial office first.