Betaine lipids overproduced in seed plants are not imported into plastid membranes and promote endomembrane expansion.

IF 5.6 2区 生物学 Q1 PLANT SCIENCES
Sarah Salomon, Marion Schilling, Catherine Albrieux, Grégory Si Larbi, Pierre-Henri Jouneau, Sylvaine Roy, Denis Falconet, Morgane Michaud, Juliette Jouhet
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引用次数: 0

Abstract

Plants and algae have to adapt to environmental changes and face various stresses that negatively affect their growth and development. One common stress is phosphate (Pi) deficiency, which is often present in the environment at limiting levels. In response to Pi deficiency, these organisms increase Pi uptake and remobilize intracellular Pi. Phospholipids are degraded to provide Pi and are replaced by non-phosphorus lipids, such as glycolipids or betaine lipids. During evolution, seed plants lost the ability to synthesize betaine lipids. By expressing Bta1 genes, which are involved in the synthesis of diacylglyceryl-N,N,N-trimethyl-homoserine (DGTS), from different species, we showed that DGTS can be produced in seed plants. In Arabidopsis, expression of BTA1 under a phosphate starvation-inducible promoter resulted in limited DGTS production without having any impact on plant growth or lipid remodelling. In transient expression systems in Nicotiana benthamiana, leaves were able to accumulate DGTS to up to 30% of their glycerolipid content at a slight expense to galactolipid and phospholipid production. At the subcellular level, we showed that DGTS is absent from plastids and seems to be enriched in endomembranes, inducing endoplasmic reticulum membrane proliferation. Finally, the DGTS synthesis pathway seems to compete with phosphatidylcholine (PC) synthesis via the Kennedy pathway but does not appear to be derived from the PC diacylglycerol backbone and therefore does not interfere with the eukaryotic pathway involved in galactolipid synthesis.

种子植物中过量产生的甜菜碱脂类被排除在质体膜之外,并促进内膜扩张。
植物和藻类必须适应环境变化,并面临各种对其生长和发育产生负面影响的压力。其中一种常见的压力是磷酸盐(Pi)缺乏,而环境中的磷酸盐经常处于极限水平。为应对 Pi 缺乏,这些生物会增加对 Pi 的吸收,并将细胞内的 Pi 重新固定。磷脂被降解以提供π,并被非磷脂(如糖脂或甜菜碱脂)取代。在进化过程中,种子植物失去了合成甜菜碱脂质的能力。通过表达不同物种中参与合成二酰甘油-N,N,N-三甲基高丝氨酸(DGTS)的 BTA1 基因,我们的工作表明种子植物中可以产生 DGTS。在拟南芥中,在磷酸盐饥饿诱导启动子下表达 BTA1 会产生有限的 DGTS,而不会对植物生长或脂质重塑产生任何影响。在烟曲霉的瞬时表达系统中,叶片能够积累多达其甘油酯含量 30% 的 DGTS,而半乳糖脂和磷脂的产生则略有减少。在亚细胞水平上,我们发现质体中没有 DGTS,而内膜中似乎富集了 DGTS,从而推动了 ER 膜的增殖。最后,DGTS 的合成途径似乎与通过肯尼迪途径合成 PC 竞争,但似乎并非来自 PC 的二酰甘油骨架,因此不会干扰半乳糖脂合成的真核途径。
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来源期刊
Journal of Experimental Botany
Journal of Experimental Botany 生物-植物科学
CiteScore
12.30
自引率
4.30%
发文量
450
审稿时长
1.9 months
期刊介绍: The Journal of Experimental Botany publishes high-quality primary research and review papers in the plant sciences. These papers cover a range of disciplines from molecular and cellular physiology and biochemistry through whole plant physiology to community physiology. Full-length primary papers should contribute to our understanding of how plants develop and function, and should provide new insights into biological processes. The journal will not publish purely descriptive papers or papers that report a well-known process in a species in which the process has not been identified previously. Articles should be concise and generally limited to 10 printed pages.
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