Molecular investigation of Coxiella burnetii, Brucella spp., Ehrlichia spp., and Borrelia spp. among patients suspected of having Crimean-Congo Hemorrhagic Fever in Iran

IF 4.7 3区医学 Q1 INFECTIOUS DISEASES

Journal of Infection and Public Health Pub Date : 2024-10-29 DOI:10.1016/j.jiph.2024.102582

Safoura Moradkasani , Mina Latifian , Mostafa Salehi-Vaziri , Fahimeh Bagheri Amiri , Ehsan Mostafavi , Ahmad Ghasemi , Saber Esmaeili

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Abstract

Objectives

Crimean-Congo Hemorrhagic Fever (CCHF) is a tick-borne zoonotic viral disease that could be a public health concern. The overlapping of clinical symptoms of some acute bacterial febrile diseases with CCHF is of importance for clinical diagnosis. This study aimed to molecularly examination of Brucella, Coxiella burnetii, Borrelia, and Ehrlichia infections among individuals suspected of CCHF in Iran.

Methods

In this study, 260 serum samples of suspected cases of CCHF with definitively negative laboratory test results for CCHF virus infection, were examined for Brucella spp., Coxiella burnetii, Borrelia spp., and Ehrlichia spp. by Real-time PCR.

Results

According to the results, 3.46 % and 3.07 % of the patients were positive for brucellosis and Q fever, respectively. Notably, no cases of borreliosis or ehrlichiosis were detected. Among the positive cases for brucellosis (N = 9), three cases were identified as Brucella abortus infection. Individuals under the age of 43 displayed a significantly higher positivity rate for Q fever (p < 0.01). Furthermore, patients presenting with chills had a 5.81-fold increased likelihood of being infected with Q fever (95 % CI: 1.39–24.26) compared to those without chills. Notably, no other variables demonstrated a statistically significant association with Q fever infection.

Discussion and conclusions

The results of this study showed that bacterial infections such as Q fever and brucellosis should be considered as differential diagnoses of CCHF. It is recommended that other bacterial infections that can cause early clinical symptoms similar to CCHF should also be taken into consideration in future studies and serological and molecular investigations of these infections should be tested on a wide scale.

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伊朗克里米亚-刚果出血热疑似患者中烧伤科克西氏菌、布鲁氏菌属、埃立克氏菌属和包柔氏菌属的分子调查。

目标：克里米亚-刚果出血热（CCHF克里米亚-刚果出血热（CCHF）是一种蜱传人畜共患病毒性疾病，可能会引发公共卫生问题。一些急性细菌性发热疾病的临床症状与克里米亚-刚果出血热重叠，这对临床诊断具有重要意义。本研究旨在对伊朗疑似 CCHF 患者的布鲁氏菌、烧伤克西氏菌、鲍氏菌和埃利希氏菌感染进行分子检测：在这项研究中，对 260 例疑似 CCHF 病例的血清样本进行了实时 PCR 检测，这些样本的 CCHF 病毒感染实验室检测结果均为阴性：结果显示，分别有 3.46% 和 3.07% 的患者对布鲁氏杆菌病和 Q 热呈阳性反应。值得注意的是，没有发现包虫病或埃希氏病病例。在布鲁氏菌病阳性病例（9 例）中，有 3 例被确定为流产布鲁氏菌感染。43 岁以下人群的 Q 热阳性率明显更高（p 讨论和结论：本研究结果表明，Q 热和布鲁氏菌病等细菌感染应被视为慢性阻塞性肺病的鉴别诊断。建议在今后的研究中也应考虑到其他可引起与 CCHF 相似的早期临床症状的细菌感染，并对这些感染进行广泛的血清学和分子学检测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Infection and Public Health PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH -INFECTIOUS DISEASES

CiteScore

13.10

自引率

1.50%

发文量

203

审稿时长

96 days

期刊介绍： The Journal of Infection and Public Health, first official journal of the Saudi Arabian Ministry of National Guard Health Affairs, King Saud Bin Abdulaziz University for Health Sciences and the Saudi Association for Public Health, aims to be the foremost scientific, peer-reviewed journal encompassing infection prevention and control, microbiology, infectious diseases, public health and the application of healthcare epidemiology to the evaluation of health outcomes. The point of view of the journal is that infection and public health are closely intertwined and that advances in one area will have positive consequences on the other. The journal will be useful to all health professionals who are partners in the management of patients with communicable diseases, keeping them up to date. The journal is proud to have an international and diverse editorial board that will assist and facilitate the publication of articles that reflect a global view on infection control and public health, as well as emphasizing our focus on supporting the needs of public health practitioners. It is our aim to improve healthcare by reducing risk of infection and related adverse outcomes by critical review, selection, and dissemination of new and relevant information in the field of infection control, public health and infectious diseases in all healthcare settings and the community.