Profiling immune cell tissue niches in the spatial -omics era.

IF 11.4 1区 医学 Q1 ALLERGY
Colin Y C Lee, James McCaffrey, Dominic McGovern, Menna R Clatworthy
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引用次数: 0

Abstract

Immune responses require complex, spatially coordinated interactions between immune cells and their tissue environment. For decades, we have imaged tissue sections to visualize a limited number of immune-related macromolecules in situ, functioning as surrogates for cell types or processes of interest. However, this inevitably provides a limited snapshot of the tissue's immune landscape. Recent developments in high-throughput spatial -omics technologies, particularly spatial transcriptomics, and its application to human samples has facilitated a more comprehensive understanding of tissue immunity by mapping fine-grained immune cell states to their precise tissue location while providing contextual information about their immediate cellular and tissue environment. These data provide opportunities to investigate mechanisms underlying the spatial distribution of immune cells and its functional implications, including the identification of immune niches, although the criteria used to define this term have been inconsistent. Here, we review recent technological and analytic advances in multiparameter spatial profiling, focusing on how these methods have generated new insights in translational immunology. We propose a 3-step framework for the definition and characterization of immune niches, which is powerfully facilitated by new spatial profiling methodologies. Finally, we summarize current approaches to analyze adaptive immune repertoires and lymphocyte clonal expansion in a spatially resolved manner.

剖析空间 "全息 "时代的免疫细胞组织龛位。
免疫反应需要免疫细胞与其组织环境之间复杂、空间协调的相互作用。几十年来,我们对组织切片进行成像,以原位观察数量有限的免疫相关大分子,作为细胞类型或相关过程的替代物。然而,这不可避免地只能提供组织免疫环境的有限快照。高通量空间 "奥米克 "技术,特别是空间转录组学的最新发展及其在人体样本中的应用,通过将精细的免疫细胞状态映射到其精确的组织位置,同时提供有关其直接细胞和组织环境的背景信息,促进了对组织免疫的更全面了解。这些数据为研究免疫细胞的空间分布机制及其功能影响提供了机会,包括识别 "免疫龛",尽管用于定义这一术语的标准并不一致。在此,我们回顾了多参数空间剖析技术和分析方面的最新进展,重点关注这些方法如何在转化免疫学中产生新的见解。我们提出了定义和描述免疫龛位的三步框架,新的空间剖析方法有力地促进了这一工作。最后,我们总结了目前以空间分辨方式分析适应性免疫复合物和淋巴细胞克隆扩增的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
25.90
自引率
7.70%
发文量
1302
审稿时长
38 days
期刊介绍: The Journal of Allergy and Clinical Immunology is a prestigious publication that features groundbreaking research in the fields of Allergy, Asthma, and Immunology. This influential journal publishes high-impact research papers that explore various topics, including asthma, food allergy, allergic rhinitis, atopic dermatitis, primary immune deficiencies, occupational and environmental allergy, and other allergic and immunologic diseases. The articles not only report on clinical trials and mechanistic studies but also provide insights into novel therapies, underlying mechanisms, and important discoveries that contribute to our understanding of these diseases. By sharing this valuable information, the journal aims to enhance the diagnosis and management of patients in the future.
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