Gut microbiota and graft-versus-host disease in hematopoietic stem cell transplant patients.

IF 1.3 Q4 MICROBIOLOGY

Iranian Journal of Microbiology Pub Date : 2024-10-01 DOI:10.18502/ijm.v16i5.16800

Pegah Panahi, Amir Hossein Hashemian, Mehrdad Payandeh, Mahdi Taghadosi, Bizhan Nomanpour

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Abstract

Background and objectives: Graft-versus-host disease (GvHD) frequently complicates hematopoietic stem cell transplantation (HSCT). Emerging evidence suggests a correlation between gut microbiota and GvHD risk. This study aims to elucidate the microbiota profiles in HSCT patients before and after transplantation and their association with GvHD.

Materials and methods: This study, conducted from December 2022 to December 2023, involved the collection of 15 stool samples from HSCT patients. Bacterial content was quantified using real-time PCR, while interleukin-6 levels were assessed via ELISA.

Results: Among the 15 participants (8 male, 7 female), 9 underwent allogeneic HSCT (allo-HSCT) and 6 received autologous HSCT. In the aGvHD group, there was a significant reduction in the abundance of Bacteroides and Bifidobacterium compared to those without aGvHD. Additionally, declines were observed in Clostridium and Firmicutes populations. The genus Blautia also showed reduced prevalence in the aGvHD group, whereas no significant differences were noted in the uncomplicated group. ELISA analysis revealed that interleukin-6 levels remained within the normal range (30-960 pg/ml) with no significant elevation in the aGvHD group.

Conclusion: The study highlights a notable association between alterations in gut microbiota, specifically reductions in certain bacterial populations and the development of aGvHD following allo-HSCT.

查看原文本刊更多论文

造血干细胞移植患者的肠道微生物群与移植物抗宿主疾病。

背景与目的：移植物抗宿主疾病（GvHD）经常并发造血干细胞移植（HSCT）。新的证据表明，肠道微生物群与移植物抗宿主疾病风险之间存在相关性。本研究旨在阐明造血干细胞移植患者在移植前后的微生物群谱及其与GvHD的关系：本研究于 2022 年 12 月至 2023 年 12 月进行，收集了造血干细胞移植患者的 15 份粪便样本。使用实时 PCR 对细菌含量进行量化，同时通过 ELISA 评估白细胞介素-6 的水平：15名参与者（8男7女）中，9人接受了异基因造血干细胞移植（allo-HSCT），6人接受了自体造血干细胞移植。与未接受异基因造血干细胞移植的患者相比，接受异基因造血干细胞移植的患者体内乳酸杆菌和双歧杆菌的数量明显减少。此外，梭状芽孢杆菌（Clostridium）和真菌（Firmicutes）的数量也有所下降。布劳氏菌属在 aGvHD 组中的流行率也有所下降，而在无并发症组中则无明显差异。ELISA 分析显示，白细胞介素-6 水平仍在正常范围内（30-960 pg/ml），无并发症组的白细胞介素-6 水平没有明显升高：结论：这项研究强调了肠道微生物群的改变，特别是某些细菌群的减少与allo-HSCT后发生aGvHD之间的明显联系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Journal of Microbiology MICROBIOLOGY-

CiteScore

2.40

自引率

7.10%

发文量

审稿时长

12 weeks

期刊介绍： The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.