Uncovering the Potential Somatic Angiotensin-Converting Enzyme (sACE) Inhibitory Capacity of Peptides from Acheta domesticus: Insights from In Vitro Gastrointestinal Digestion.

IF 4.7 2区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Foods Pub Date : 2024-10-29 DOI:10.3390/foods13213462

Carla S S Teixeira, Bruno Carriço-Sá, Caterina Villa, Joana Costa, Isabel Mafra, Isabel M P L V O Ferreira, Miguel A Faria, Tânia G Tavares

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引用次数: 0

Abstract

Entomophagy is being proposed as a sustainable and nutritious alternative protein source. Additionally, insect consumption is also associated with some health benefits mediated by bioactive compounds produced during gastrointestinal (GI) digestion. The antihypertensive property resulting from the inhibition of the somatic angiotensin-converting enzyme (sACE) by small peptides is one of the most common bioactivities related to insect consumption. This study aimed to investigate the potential sACE-inhibitory capacity of six peptides (AVQPCF, CAIAW, IIIGW, QIVW, PIVCF, and DVW), previously identified by the in silico GI digestion of Acheta domesticus proteins, validate their formation after in vitro GI digestion of A. domesticus by LC-MS/MS, and assess the bioactivity of the bioaccessible digesta. The results showed that the IC₅₀ values of AVQPCF, PIVCF, and CAIAW on sACE were 3.69 ± 0.25, 4.63 ± 0.16, and 6.55 ± 0.52 μM, respectively. The obtained digesta demonstrated a sACE-inhibitory capacity of 77.1 ± 11.8 µg protein/mL extract (IC₅₀). This is the first report of the sACE-inhibitory capacity attributed to whole A. domesticus subjected to GI digestion without any pre-treatment or protein concentration. This evidence highlights the potential antihypertensive effect of both the digesta and the identified peptides.

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揭示豚鼠肽潜在的体细胞血管紧张素转换酶（sACE）抑制能力：体外胃肠消化的启示。

人们建议将昆虫膳食作为一种可持续的营养替代蛋白质来源。此外，食用昆虫还与胃肠道（GI）消化过程中产生的生物活性化合物所带来的一些健康益处有关。小肽对体细胞血管紧张素转换酶（sACE）的抑制作用所产生的抗高血压特性是与食用昆虫有关的最常见的生物活性之一。本研究旨在研究之前通过对家蝇蛋白的硅学消化鉴定出的六种肽（AVQPCF、CAIAW、IIIGW、QIVW、PIVCF 和 DVW）的潜在 sACE 抑制能力，通过 LC-MS/MS 验证它们在家蝇体外消化后的形成，并评估生物可吸收消化物的生物活性。结果表明，AVQPCF、PIVCF 和 CAIAW 对 sACE 的 IC50 值分别为 3.69 ± 0.25、4.63 ± 0.16 和 6.55 ± 0.52 μM。获得的消化液对 sACE 的抑制能力为 77.1 ± 11.8 µg 蛋白/毫升提取物（IC50）。这是首次报道在未经任何预处理或蛋白质浓缩的情况下，整个驯鹿消化液对 sACE 的抑制能力。这一证据凸显了消化液和已鉴定肽的潜在降压作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Foods Immunology and Microbiology-Microbiology

CiteScore

7.40

自引率

15.40%

发文量

3516

审稿时长

15.83 days

期刊介绍： Foods (ISSN 2304-8158) is an international, peer-reviewed scientific open access journal which provides an advanced forum for studies related to all aspects of food research. It publishes reviews, regular research papers and short communications. Our aim is to encourage scientists, researchers, and other food professionals to publish their experimental and theoretical results in as much detail as possible or share their knowledge with as much readers unlimitedly as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. There are, in addition, unique features of this journal: manuscripts regarding research proposals and research ideas will be particularly welcomed electronic files or software regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material we also accept manuscripts communicating to a broader audience with regard to research projects financed with public funds