Ying Huang , Li-Fan Cui , Rui Shen , Ding-Yi Chen , Min Jin , Xue Jiao , Yu-Guang Chen , Ming-Xuan Pan , Ya-Dong Hu , Zhe Zhao
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引用次数: 0
Abstract
Galectins belong to a family of galactoside-binding proteins and exhibit diverse biological functions. In the present research, a tandem-repeat type galectin (named ToGalectin) was identified from obscure puffer Takifugu obscurus. The 296 amino acids ToGalectin contained two carbohydrate recognition domains (CRDs), one of which possessed two conserved carbohydrate binding motifs. Phylogenetic analysis showed that ToGalectin clustered tightly with other galectin-8 proteins from teleost fish. ToGalectin transcripts were ubiquitously expressed in all tissues examined and its expression was significantly upregulated in the liver, kidney, and intestine after Vibrio harveyi or Staphylococcus aureus infection. To investigate the effect of carbohydrate binding sites on biological activity, ToGalectin and its mutant (MUT-ToGalectin) were expressed and purified. The recombinant ToGalectin and MUT-ToGalectin proteins showed strong agglutinating activity against both V. harveyi and S. aureus. rToGalectin could bind to all tested carbohydrates and bacteria, whereas rMUT-ToGalectin bound to some carbohydrates and bacteria with specific and relatively strong affinity. rToGalectin significantly suppressed the growth of all six bacteria detected and promoted bacterial clearance in vivo, whereas MUT-ToGalectin inhibited the growth of only two bacterial species, which could be attributed to the differences in conserved motifs within the CRDs. Our results suggested that ToGalectin is involved in the immune response against bacterial infection and the clearance of pathogens in T. obscurus.
期刊介绍:
Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.