Enhanced activity of mixed-culture electroactive biofilms and sulfamethoxazole removal efficiency by adding N-acyl-homoserine lactones in bio-electrochemical system.

Abstract

The addition of exogenous quorum sensing signaling molecules significantly enhanced the degradation efficiency of antibiotics, such as chloramphenicol in bio-electrochemical systems (BESs). However, the effects and mechanisms by which AHLs addition in BES facilitated the removal of sulfamethoxazole (SMX) remained inadequately explored. This study systematically compared the electrochemical performance and SMX removal efficiency in BES under two conditions: with and without the addition of N-acyl-homoserine lactones (AHLs) signaling molecules. In comparison to the control group, the AHL-treated group exhibited an increase in maximum output voltage from 340 to 489.67 mV, alongside a notable enhancement in SMX removal efficiency over 120 h ranging from 14.65% to 15.76%. Analyses of the live and dead cells and extracellular polymeric substances (EPS) composition revealed that following AHLs addition, both the ratio of live to dead cells and protein content within EPS increased by 12.66% and 74.37%, respectively. Furthermore, microbial community structure analysis indicated that after AHLs supplementation, there was a marked increase in the abundance of electroactive microorganisms as well as antibiotic-degrading and nitrogen-removing bacteria. Notably, Klebsiella - characterised by its electroactivity along with antibiotic degradation and nitrogen removal capabilities - exhibited a relative abundance reaching 56.84% in AHL, reflecting an increase of 28.31% compared to Blank; additionally, electroactive bacteria Dysgonomonas showed a relative abundance rise of 2.49%. Collectively, these findings suggested that enhancements in SMX removal efficiency upon AHLs addition were primarily driven by improvements in electrochemical performance coupled with alterations in microbial community structure.