Virulence Profiles of Salmonella enterica Isolated from Three Food Matrices Collected from Retail Markets.

IF 1.9 2区农林科学 Q3 FOOD SCIENCE & TECHNOLOGY

Foodborne pathogens and disease Pub Date : 2024-11-11 DOI:10.1089/fpd.2024.0104

Sandy V Alarcón Navas, Eliana M Pereira Cardeño, María F Martínez, Nicolás F Ortiz Suárez, Alexander David Castro, Ruth A Martínez-Vega, Marcela Navarro Rosado, Clara I González, Giovanna Rincón Cruz

{"title":"Virulence Profiles of Salmonella enterica Isolated from Three Food Matrices Collected from Retail Markets.","authors":"Sandy V Alarcón Navas, Eliana M Pereira Cardeño, María F Martínez, Nicolás F Ortiz Suárez, Alexander David Castro, Ruth A Martínez-Vega, Marcela Navarro Rosado, Clara I González, Giovanna Rincón Cruz","doi":"10.1089/fpd.2024.0104","DOIUrl":null,"url":null,"abstract":"Salmonella enterica is one of the most common foodborne pathogens associated with the consumption of contaminated porcine, dairy, and avian products. Nontyphoidal Salmonella is a major cause of bacterial diarrhea, responsible for ∼150 million cases and 60,000 deaths annually. The main goal of this study was to determine the prevalence of Salmonella spp. and to establish the virulence profile (VP) from genes (avrA, invE, ssaD, sseF, ssaQ, ttrC) and plasmid genes (pefA, spvB, spvC) in isolates obtained from cheese, chicken, and pork sold in food markets in Barrancabermeja, Colombia. A survey was conducted on 100 samples each matrix. The detection of Salmonella spp. followed the ISO 6579:2017 standards modified, and isolates were confirmed using the invA gene. In addition, single polymerase chain reaction assays were developed to detect the nine virulence genes. Salmonella spp. was found in 62%, 32%, and 14% of pork, chicken, and cheese samples, respectively. A total of 277 isolates were biochemically, serologically, and molecularly compatible with Salmonella spp. The most representative serogroups were C and B. Forty-seven combinations of virulence gene were detected; 53.5% of the pork isolates, 46.2% of the cheese isolates, and 39% of the chicken isolates were distributed among VP1, VP2, and VP3 suggesting a higher pathogenic potential. In addition, seven isolates harbored plasmid-encoded virulence genes (spvB and spvC), which are associated with increased invasiveness. The results revealed a higher prevalence of Salmonella spp. in pork and chicken compared with other studies conducted in Colombia. The serogroups identified include serovars that more frequently affect humans Salmonella Enteriditis, Salmonella Newport, and Salmonella Typhimurium. The isolations have the majority of the virulence genes studied. These findings highlight the need to improve control measures and educate food handlers to minimize the presence of Salmonella spp. and its potential transmission.","PeriodicalId":12333,"journal":{"name":"Foodborne pathogens and disease","volume":" ","pages":""},"PeriodicalIF":1.9000,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Foodborne pathogens and disease","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1089/fpd.2024.0104","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Salmonella enterica is one of the most common foodborne pathogens associated with the consumption of contaminated porcine, dairy, and avian products. Nontyphoidal Salmonella is a major cause of bacterial diarrhea, responsible for ∼150 million cases and 60,000 deaths annually. The main goal of this study was to determine the prevalence of Salmonella spp. and to establish the virulence profile (VP) from genes (avrA, invE, ssaD, sseF, ssaQ, ttrC) and plasmid genes (pefA, spvB, spvC) in isolates obtained from cheese, chicken, and pork sold in food markets in Barrancabermeja, Colombia. A survey was conducted on 100 samples each matrix. The detection of Salmonella spp. followed the ISO 6579:2017 standards modified, and isolates were confirmed using the invA gene. In addition, single polymerase chain reaction assays were developed to detect the nine virulence genes. Salmonella spp. was found in 62%, 32%, and 14% of pork, chicken, and cheese samples, respectively. A total of 277 isolates were biochemically, serologically, and molecularly compatible with Salmonella spp. The most representative serogroups were C and B. Forty-seven combinations of virulence gene were detected; 53.5% of the pork isolates, 46.2% of the cheese isolates, and 39% of the chicken isolates were distributed among VP1, VP2, and VP3 suggesting a higher pathogenic potential. In addition, seven isolates harbored plasmid-encoded virulence genes (spvB and spvC), which are associated with increased invasiveness. The results revealed a higher prevalence of Salmonella spp. in pork and chicken compared with other studies conducted in Colombia. The serogroups identified include serovars that more frequently affect humans Salmonella Enteriditis, Salmonella Newport, and Salmonella Typhimurium. The isolations have the majority of the virulence genes studied. These findings highlight the need to improve control measures and educate food handlers to minimize the presence of Salmonella spp. and its potential transmission.

查看原文本刊更多论文

从零售市场收集的三种食物基质中分离出的肠炎沙门氏菌的毒力谱。

肠炎沙门氏菌是最常见的食源性病原体之一，与食用受污染的猪肉、奶制品和禽产品有关。非伤寒沙门氏菌是细菌性腹泻的主要病因，每年造成 1.5 亿例病例和 6 万人死亡。本研究的主要目的是确定沙门氏菌属的流行率，并根据从哥伦比亚巴兰卡韦梅哈食品市场出售的奶酪、鸡肉和猪肉中分离的菌株中获得的基因（avrA、invE、ssaD、seF、ssaQ、ttrC）和质粒基因（pefA、spvB、spvC）建立毒力谱（VP）。对每种基质的 100 个样本进行了调查。沙门氏菌属的检测按照 ISO 6579:2017 标准进行了修改，并使用 invA 基因对分离物进行了确认。此外，还开发了单聚合酶链式反应检测方法来检测九种毒力基因。在猪肉、鸡肉和奶酪样本中发现的沙门氏菌分别占 62%、32% 和 14%。共检测到 47 种毒力基因组合；53.5% 的猪肉分离物、46.2% 的奶酪分离物和 39% 的鸡肉分离物的毒力基因分布在 VP1、VP2 和 VP3 之间，这表明沙门氏菌具有较高的致病性。此外，7 个分离物携带质粒编码的毒力基因（spvB 和 spvC），这与入侵性增加有关。研究结果表明，与哥伦比亚进行的其他研究相比，猪肉和鸡肉中沙门氏菌属的流行率较高。所发现的血清群包括更常影响人类的血清型：肠炎沙门氏菌、新港沙门氏菌和鼠伤寒沙门氏菌。这些分离菌株具有所研究的大多数毒力基因。这些发现突出表明，有必要改进控制措施，并对食品处理人员进行教育，以尽量减少沙门氏菌的存在及其潜在的传播。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Foodborne pathogens and disease 医学-食品科技

CiteScore

5.30

自引率

3.60%

发文量

审稿时长

1 months

期刊介绍： Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide. Foodborne Pathogens and Disease coverage includes: Agroterrorism Safety of organically grown and genetically modified foods Emerging pathogens Emergence of drug resistance Methods and technology for rapid and accurate detection Strategies to destroy or control foodborne pathogens Novel strategies for the prevention and control of plant and animal diseases that impact food safety Biosecurity issues and the implications of new regulatory guidelines Impact of changing lifestyles and consumer demands on food safety.