Qiongyao Zeng , Yiyang Tang , Yujun Liu , Ye Yang , Pingyuan Li , Zejun Zhou , Qinbo Qin
{"title":"A recombinant sPLA2 protein promotes gut mucosal barrier against bacterial infection in fish","authors":"Qiongyao Zeng , Yiyang Tang , Yujun Liu , Ye Yang , Pingyuan Li , Zejun Zhou , Qinbo Qin","doi":"10.1016/j.dci.2024.105288","DOIUrl":null,"url":null,"abstract":"<div><div>Secreted phospholipase A2 family protein (sPLA2) is associated with immune response and plays a critical role in the regulation of gut homeostasis. However, whether sPLA2 is involved in innate immunity in teleost is essentially unknown. For this purpose, we reported the identification of a classical sPLA2 in grass carp (<em>Ci</em>sPLA2) and elucidated its role in the antibacterial immunity in this study. The result of bioinformatics analysis showed that mammalian sPLA2-IIA is the most similar homologue to <em>Ci</em>sPLA2. <em>CisPLA2</em> is expressed in a variety of tissues, including liver and gut, and is significantly upregulated in response to <em>Aeromonas hydrophila</em> infection. Recombinant <em>Ci</em>sPLA2 protein (r<em>Ci</em>sPLA2) showed significant antibacterial activity against <em>A</em>. <em>hydrophila</em> by enhancing the phagocytosis of host phagocytes <em>in vitro</em>. Moreover, r<em>Ci</em>sPLA2 induces significant expression of the antimicrobial molecules and tight junctions in the gut during bacterial infection. Fish administered with r<em>Ci</em>sPLA2 significantly alleviates the gut permeability and apoptosis. In addition, r<em>Ci</em>sPLA2 preserves the morphology of the gut mucosa and limits the colonization of <em>A</em>. <em>hydrophila</em> in systemic immune organs. These results indicate that <em>Ci</em>sPLA2 plays a crucial role in the regulation of gut mucosal barrier, and thus has a potential application for antimicrobial immunity in fish.</div></div>","PeriodicalId":11228,"journal":{"name":"Developmental and comparative immunology","volume":"162 ","pages":"Article 105288"},"PeriodicalIF":2.7000,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Developmental and comparative immunology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0145305X24001605","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FISHERIES","Score":null,"Total":0}
引用次数: 0
Abstract
Secreted phospholipase A2 family protein (sPLA2) is associated with immune response and plays a critical role in the regulation of gut homeostasis. However, whether sPLA2 is involved in innate immunity in teleost is essentially unknown. For this purpose, we reported the identification of a classical sPLA2 in grass carp (CisPLA2) and elucidated its role in the antibacterial immunity in this study. The result of bioinformatics analysis showed that mammalian sPLA2-IIA is the most similar homologue to CisPLA2. CisPLA2 is expressed in a variety of tissues, including liver and gut, and is significantly upregulated in response to Aeromonas hydrophila infection. Recombinant CisPLA2 protein (rCisPLA2) showed significant antibacterial activity against A. hydrophila by enhancing the phagocytosis of host phagocytes in vitro. Moreover, rCisPLA2 induces significant expression of the antimicrobial molecules and tight junctions in the gut during bacterial infection. Fish administered with rCisPLA2 significantly alleviates the gut permeability and apoptosis. In addition, rCisPLA2 preserves the morphology of the gut mucosa and limits the colonization of A. hydrophila in systemic immune organs. These results indicate that CisPLA2 plays a crucial role in the regulation of gut mucosal barrier, and thus has a potential application for antimicrobial immunity in fish.
期刊介绍:
Developmental and Comparative Immunology (DCI) is an international journal that publishes articles describing original research in all areas of immunology, including comparative aspects of immunity and the evolution and development of the immune system. Manuscripts describing studies of immune systems in both vertebrates and invertebrates are welcome. All levels of immunological investigations are appropriate: organismal, cellular, biochemical and molecular genetics, extending to such fields as aging of the immune system, interaction between the immune and neuroendocrine system and intestinal immunity.