Jessica Santos Passos Costa, Heli Vieira Brandão, Mara Viana Cardoso Amaral, Gabriela Cintra Dos Santos, Camilla da Cruz Martins, Michelle de Santana Xavier Ramos, Tatiana de Oliveira Vieira, Raquel Guimarães Benevides, Graciete Oliveira Vieira
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引用次数: 0
Abstract
Background: The newborn's intestinal microbiota can vary with gestational age. Reliable analyses of stool samples require establishing the steps of collection, packaging, transportation, and storage.
Objective: To describe the development of a protocol and test an algorithm for the sequence of actions and procedures for the collection, packaging, transportation, and storage of stool samples from Preterm Newborn (PTNB) admitted to a Neonatal Intensive Care Unit (NICU).
Materials and methods: This is a descriptive, observational study linked to a non-randomized controlled clinical trial conducted in the NICU of two public hospitals covenanted to the Unified Health System of a large city in inland northeastern Brazil. The methodology adopted in its development followed the Brazilian Ministry of Health Preparation Guide recommendations.
Results: The flow of actions and procedures was consistent with scientific evidence. The sequence of work process steps to implement the proposed protocol was tested and resulted in a graphical representation of an algorithm compatible with the reality of Brazilian public hospitals.
Conclusion: Following the protocol steps with the description of the rules of conduct and recommendations regarding the collection, packaging, transportation, and storage of stool samples from PTNBs ensured the preservation and integrity of the bacterial DNA in the stools sample. The details of the recommendations will allow their reproducibility and improvement by professionals and researchers with similar study objects.
Trial registration: World Health Organization (WHO) under Universal Trial Number (UTN) code U1111-1266-2295, under register RBR-3mm7cs in the Brazilian Registry of Clinical Trials (REBEC).
背景：新生儿的肠道微生物群会随着胎龄的变化而变化。对粪便样本进行可靠的分析需要确定收集、包装、运输和储存的步骤：描述早产新生儿（PTNB）在新生儿重症监护病房（NICU）的粪便样本收集、包装、运输和储存的操作顺序和程序的协议制定和算法测试：这是一项与非随机对照临床试验相关的描述性观察研究，在巴西东北部内陆一个大城市的两家隶属于统一卫生系统的公立医院的新生儿重症监护室进行。研究方法遵循了巴西卫生部《准备指南》的建议：结果：行动和程序的流程符合科学依据。对实施建议规程的工作流程步骤顺序进行了测试，得出了符合巴西公立医院实际情况的图解算法：结论：按照规程中关于 PTNB 粪便样本的收集、包装、运输和储存的行为规则和建议所描述的步骤进行操作，可确保粪便样本中细菌 DNA 的保存和完整性。这些建议的细节将使专业人员和研究人员在进行类似研究时具有可重复性和改进性：世界卫生组织（WHO）通用试验编号（UTN）U1111-1266-2295，巴西临床试验登记处（REBEC）登记号RBR-3mm7cs。
期刊介绍：
CLINICS is an electronic journal that publishes peer-reviewed articles in continuous flow, of interest to clinicians and researchers in the medical sciences. CLINICS complies with the policies of funding agencies which request or require deposition of the published articles that they fund into publicly available databases. CLINICS supports the position of the International Committee of Medical Journal Editors (ICMJE) on trial registration.
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