Salmonella enterica Enteritidis and Heidelberg serotype-specific molecular detection in poultry samples by a rapid isothermal method.

IF 1.6 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

British Poultry Science Pub Date : 2024-11-11 DOI:10.1080/00071668.2024.2419623

C F D S Andrade, M N Souza, I I D S Dantas, A S K Fonseca, N Ikuta, D Kipper, V R Lunge

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Abstract

1. Loop-mediated isothermal amplification (LAMP) assays were developed to detect Salmonella enterica subspecies enterica serotypes Enteritidis and Heidelberg in poultry farms. These serotype-specific methods were evaluated in comparison with PCR in the analysis of different Salmonella spp. serotypes from a culture collection and poultry farm samples.2. The results demonstrated the specific amplification of the genetic targets safA in all S. Enteritidis (n = 10) and ACF69659 in all S. Heidelberg (n = 36) isolates from the culture collection. The remaining isolates from other Salmonella spp. serotypes (n = 84) and bacterial species (n = 8) were negative in both LAMP assays.3. The methods detected DNAs from S. Enteritidis and S. Heidelberg after a single-step pre-enrichment in buffered peptone water of the poultry samples, which agreed with previously developed PCR methods to detect these same two serotypes.4. In conclusion, LAMP assays were useful for rapid serotype-specific detection, being suitable for surveillance purposes in resource-limited environments such as poultry farms.

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用快速等温法对家禽样本中的肠炎沙门氏菌和海德堡血清型进行特异性分子检测。

1.开发了环路介导等温扩增（LAMP）检测方法，用于检测家禽养殖场中的肠炎沙门氏菌亚种和海德堡沙门氏菌血清型。结果表明，在所有肠炎沙门氏菌（n = 10）和所有海德堡沙门氏菌（n = 36）的培养物分离物中，均能特异性扩增基因靶标 safA。3. 在家禽样本的缓冲蛋白胨水中进行单步预富集后，这些方法检测到了肠炎沙门氏菌和海德堡沙门氏菌的 DNA，这与之前开发的检测这两种血清型的 PCR 方法一致。 4. 总之，LAMP 检测法可用于快速检测血清型特异性，适合在家禽养殖场等资源有限的环境中进行监测。

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来源期刊

British Poultry Science 农林科学-奶制品与动物科学

CiteScore

3.90

自引率

5.00%

发文量

审稿时长

4.5 months

期刊介绍： From its first volume in 1960, British Poultry Science has been a leading international journal for poultry scientists and advisers to the poultry industry throughout the world. Over 60% of the independently refereed papers published originate outside the UK. Most typically they report the results of biological studies with an experimental approach which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: anatomy, embryology, biochemistry, biophysics, physiology, reproduction and genetics, behaviour, microbiology, endocrinology, nutrition, environmental science, food science, feeding stuffs and feeding, management and housing welfare, breeding, hatching, poultry meat and egg yields and quality.Papers that adopt a modelling approach or describe the scientific background to new equipment or apparatus directly relevant to the industry are also published. The journal also features rapid publication of Short Communications. Summaries of papers presented at the Spring Meeting of the UK Branch of the WPSA are published in British Poultry Abstracts .