Virus susceptibility of a new cell line derived from the muscle of koi (Cyprinus carpio koi)

IF 2.5 4区医学 Q3 VIROLOGY

Archives of Virology Pub Date : 2024-11-14 DOI:10.1007/s00705-024-06168-3

Hongli Jing, Lin Mei, Jizhou Lv, Min Zhang, Na Wang, Lipu Xu, Shaoqiang Wu

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Abstract

In this study, a continuous cell line (KM cells) derived from koi (Cyprinus carpio koi) muscle was established and characterized. The KM cells were subcultured for more than 70 passages and showed high viability after long-term cryopreservation. The KM cell line was optimally cultured in medium 199 containing 10% foetal bovine serum at 25°C. A chromosome analysis indicated that the cell line remained diploid, with a mean chromosome count of 100. DNA sequencing and comparative analysis of the 16S rRNA and cytochrome oxidase I gene sequences showed that the KM cell line originated from koi. In transfection experiments using the plasmid pEGFP, KM cells demonstrated a high level of transfection efficiency, suggesting their potential for use in foreign gene expression studies. Inoculation with spring viraemia of carp virus (SVCV) resulted in a substantial cytopathic effect, and the level of production of SVCV in KM cells was higher than that in the epithelioma papulosum cyprinid (EPC) cell line that is normally used to produce the virus. However, no cytopathic effect was observed when these cells were inoculated with koi herpesvirus, carp oedema virus, or grass carp reovirus. These observations suggest that the newly established KM cell line will be a valuable tool for investigating the pathogenesis of infection with spring viraemia of carp virus.

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从锦鲤（Cyprinus carpio koi）肌肉中提取的新细胞系对病毒的敏感性。

本研究建立并鉴定了源自锦鲤（Cyprinus carpio koi）肌肉的连续细胞系（KM 细胞）。KM 细胞经过 70 多个传代培养，在长期冷冻保存后显示出很高的存活率。KM 细胞系在含有 10%胎牛血清的 199 培养基中培养，温度为 25°C，效果最佳。染色体分析表明，该细胞系仍为二倍体，平均染色体数为 100。对 16S rRNA 和细胞色素氧化酶 I 基因序列进行的 DNA 测序和比较分析表明，KM 细胞系源自锦鲤。在使用质粒 pEGFP 的转染实验中，KM 细胞表现出很高的转染效率，这表明它们具有用于外来基因表达研究的潜力。接种鲤鱼春季病毒血症（SVCV）会导致严重的细胞病理效应，KM 细胞产生 SVCV 的水平高于通常用于产生病毒的鲤鱼上皮瘤乳头状瘤（EPC）细胞系。然而，当这些细胞接种锦鲤疱疹病毒、鲤鱼水肿病毒或草鱼再病毒时，未观察到细胞病理效应。这些观察结果表明，新建立的 KM 细胞系将成为研究鲤鱼病毒春季病毒血症感染致病机理的重要工具。

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来源期刊

Archives of Virology 医学-病毒学

CiteScore

5.10

自引率

7.40%

发文量

324

审稿时长

4.5 months

期刊介绍： Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.