Towards the Identification of New Biomarkers in Saliva and Serum for Treatment Monitoring of Equine Gastric Ulcer Syndrome: A Liquid Proteomic Approach.

IF 2.7 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animals Pub Date : 2024-10-28 DOI:10.3390/ani14213105

Alberto Muñoz-Prieto, Ivana Rubić, Dina Rešetar Maslov, Juan Carlos González-Sánchez, Vladimir Mrljak, Jose Joaquín Cerón, Sanni Hansen

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Abstract

Equine gastric ulcer syndrome (EGUS) is a common condition in horses. This study explores the use of liquid proteomics to identify new biomarkers in saliva and serum to monitor EGUS treatment. The proteomes of horses with EGUS before and after a successful treatment with omeprazole were analysed. In saliva, 503 proteins were identified, with 7 upregulated and 6 downregulated post-treatment. Among the proteins that changed, there was an increase in vimentin, linked to wound healing, and a decrease in podocalyxin, associated with tissue damage. In serum, 206 proteins were found, with significant changes in 5. Keratin type I increased, supporting epithelial integrity, whereas immunoglobulin lambda decreased, indicating a reduced immune response. Gene ontology analysis revealed a decrease in immune-related pathways after successful treatment. Overall, 13 proteins in saliva and 5 in serum showed significant changes after treatment, highlighting the differential responses of saliva and serum in EGUS. This report creates new avenues for discovering potential biomarkers to monitor EGUS treatment, which is of high importance for the management of this prevalent disease.

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鉴定唾液和血清中的新生物标记物，用于马胃溃疡综合征的治疗监测：液体蛋白质组方法

马胃溃疡综合征（EGUS）是马的一种常见病。本研究探讨了如何利用液体蛋白质组学鉴定唾液和血清中的新生物标记物，以监测EGUS的治疗情况。研究人员分析了使用奥美拉唑成功治疗EGUS前后马匹的蛋白质组。在唾液中发现了503种蛋白质，其中7种在治疗后上调，6种在治疗后下调。在发生变化的蛋白质中，与伤口愈合有关的波形蛋白有所增加，而与组织损伤有关的荚膜黏蛋白则有所减少。在血清中发现了 206 种蛋白质，其中 5 种发生了显著变化。角蛋白 I 型增加，支持上皮的完整性，而免疫球蛋白 lambda 减少，表明免疫反应减弱。基因本体分析显示，治疗成功后，免疫相关通路减少。总体而言，治疗后唾液中的 13 种蛋白质和血清中的 5 种蛋白质发生了显著变化，这凸显了唾液和血清在 EGUS 中的不同反应。该报告为发现监测 EGUS 治疗的潜在生物标记物开辟了新的途径，这对治疗这种流行性疾病具有重要意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Animals Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

4.90

自引率

16.70%

发文量

3015

审稿时长

20.52 days

期刊介绍： Animals (ISSN 2076-2615) is an international and interdisciplinary scholarly open access journal. It publishes original research articles, reviews, communications, and short notes that are relevant to any field of study that involves animals, including zoology, ethnozoology, animal science, animal ethics and animal welfare. However, preference will be given to those articles that provide an understanding of animals within a larger context (i.e., the animals'' interactions with the outside world, including humans). There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental details and/or method of study, must be provided for research articles. Articles submitted that involve subjecting animals to unnecessary pain or suffering will not be accepted, and all articles must be submitted with the necessary ethical approval (please refer to the Ethical Guidelines for more information).