The Pattern of Gene Expression (Igf Family, Muscle Growth Regulatory Factors, and Osteogenesis-Related Genes) Involved in the Growth of Skeletal Muscle in Pikeperch (Sander lucioperca) During Ontogenesis.

IF 2.7 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animals Pub Date : 2024-10-26 DOI:10.3390/ani14213089

Fatemeh Lavajoo Bolgouri, Bahram Falahatkar, Miquel Perelló-Amorós, Fatemeh Moshayedi, Iraj Efatpanah, Joaquim Gutiérrez

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引用次数: 0

Abstract

The pikeperch (Sander lucioperca) is an economically important freshwater fish and a valuable food with high market acceptance. It is undergoing important changes in growth and regulatory metabolism during the ontogeny. Hence, the current study aims to investigate the mRNA expression of the growth hormone (gh)/insulin-like growth factor (igf) axis (ghr, igfI, igfbp, igfr), muscle regulatory factors (pax7, myf5, myod, myogenin, mrf, mymk, mstn), and osteogenesis-related genes (colla1a, fib1a, on, op, ostn) from hatching through day 40th post-hatching (DPH). The average total length (TL) of larvae measured at hatching was 3.6 ± 0.4 mm (67 degree days), and at the end of the experiment (40 DPH, 777 degree days), it was 27.1 ± 1.1 mm. The results showed three phases of gene expression in day 0 (egg), larval, and juvenile stages of pikeperch, which can be a progression or transition from the initial state toward the juvenile state. The expression pattern of myf5, mymk, and fib1a genes showed the highest value at day 0. The growth hormone receptor gene (ghr) and igfbp5 were raised to 1 DPH, whereas increased expression of igfI, igfII, igf1bp4, igf1rb, myod2, and mrf4 was detected at 14 DPH. The myod1, pax7, op, ostc, on, igf1ra, and col1a1a genes were highly expressed at 21 DPH and juvenile stages. According to the PLS-DA model, the most relevant VIPs are myf5 and mymk as best markers of earlier stages and igf1ra, ostc, pax7, and ghr as markers of later stages of ontogeny. Results from this study suggest that basal metabolism, growth of body cells and muscles, and bone proliferation and development can be regulated by the dynamic changes in gene expression patterns in this species. The identified genes will help to understand the basic biological process of pikeperch larvae and development, which is very important in pikeperch farming.

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梭子鱼（Sander lucioperca）本体发育过程中涉及骨骼肌生长的基因表达模式（Igf 家族、肌肉生长调节因子和骨生成相关基因）。

梭鲈鱼（Sander lucioperca）是一种具有重要经济价值的淡水鱼，也是一种市场认可度很高的珍贵食品。它在本体发育过程中的生长和调节代谢发生了重要变化。因此，本研究旨在探讨生长激素（gh）/胰岛素样生长因子（igf）轴（ghr、igfI、igfbp、igfr）、肌肉调控因子（pax7、myf5、myod、myogenin、mrf、mymk、mstn）以及成骨相关基因（colla1a、fib1a、on、op、ostn）从孵化到孵化后第40天（DPH）的mRNA表达。幼虫孵化时的平均总长度（TL）为 3.6 ± 0.4 毫米（67 度日），实验结束时（孵化后第 40 天，777 度日）的平均总长度（TL）为 27.1 ± 1.1 毫米。结果表明，梭子鱼在第 0 天（卵）、幼鱼和幼鱼阶段有三个阶段的基因表达，这可能是从初始状态向幼鱼状态的递进或过渡。myf5、mymk和fib1a基因在第0天的表达量最高。生长激素受体基因（ghr）和igfbp5的表达提高到了1天，而igfI、igfII、igf1bp4、igf1rb、myod2和mrf4的表达则在14天时有所提高。myod1、pax7、op、ostc、on、igf1ra 和 col1a1a 基因在 21 DPH 和幼体阶段高表达。根据 PLS-DA 模型，最相关的 VIP 是 myf5 和 mymk，它们是早期阶段的最佳标记，而 igf1ra、ostc、pax7 和 ghr 则是本体后期阶段的标记。这项研究的结果表明，该物种的基础代谢、体细胞和肌肉的生长以及骨骼的增殖和发育可以通过基因表达模式的动态变化来调节。所发现的基因将有助于了解梭子鱼幼体和发育的基本生物学过程，这在梭子鱼养殖中非常重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Animals Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

4.90

自引率

16.70%

发文量

3015

审稿时长

20.52 days

期刊介绍： Animals (ISSN 2076-2615) is an international and interdisciplinary scholarly open access journal. It publishes original research articles, reviews, communications, and short notes that are relevant to any field of study that involves animals, including zoology, ethnozoology, animal science, animal ethics and animal welfare. However, preference will be given to those articles that provide an understanding of animals within a larger context (i.e., the animals'' interactions with the outside world, including humans). There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental details and/or method of study, must be provided for research articles. Articles submitted that involve subjecting animals to unnecessary pain or suffering will not be accepted, and all articles must be submitted with the necessary ethical approval (please refer to the Ethical Guidelines for more information).