{"title":"Defense-related callose synthase PMR4 promotes root hair callose deposition and adaptation to phosphate deficiency in Arabidopsis thaliana","authors":"Kentaro Okada, Koei Yachi, Tan Anh Nhi Nguyen, Satomi Kanno, Shigetaka Yasuda, Haruna Tadai, Chika Tateda, Tae-Hong Lee, Uyen Nguyen, Kanako Inoue, Natsuki Tsuchida, Taiga Ishihara, Shunsuke Miyashima, Kei Hiruma, Kyoko Miwa, Takaki Maekawa, Michitaka Notaguchi, Yusuke Saijo","doi":"10.1111/tpj.17134","DOIUrl":null,"url":null,"abstract":"<p>Plants acquire phosphorus (P) primarily as inorganic phosphate (Pi) from the soil. Under Pi deficiency, plants induce an array of physiological and morphological responses, termed phosphate starvation response (PSR), thereby increasing Pi acquisition and use efficiency. However, the mechanisms by which plants adapt to Pi deficiency remain to be elucidated. Here, we report that deposition of a β-1,3-glucan polymer called callose is induced in <i>Arabidopsis thaliana</i> root hairs under Pi deficiency, in a manner independent of PSR-regulating <i>PHR1/PHL1</i> transcription factors and <i>LPR1/LPR2</i> ferroxidases. Genetic studies revealed <i>PMR4</i> (<i>GSL5</i>) callose synthase being required for the callose deposition in Pi-depleted root hairs. Loss of <i>PMR4</i> also reduces Pi acquisition in shoots and plant growth under low Pi conditions. The defects are not recovered by simultaneous disruption of <i>SID2</i>, mediating defense-associated salicylic acid (SA) biosynthesis, excluding SA defense activation from the cause of the observed <i>pmr4</i> phenotypes. Grafting experiments and characterization of plants expressing <i>PMR4</i> specifically in root hair cells suggest that a PMR4 pool in the cell type contributes to shoot growth under Pi deficiency. Our findings thus suggest an important role for <i>PMR4</i> in plant adaptation to Pi deficiency.</p>","PeriodicalId":233,"journal":{"name":"The Plant Journal","volume":"120 6","pages":"2639-2655"},"PeriodicalIF":6.2000,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/tpj.17134","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Plant Journal","FirstCategoryId":"2","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/tpj.17134","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Plants acquire phosphorus (P) primarily as inorganic phosphate (Pi) from the soil. Under Pi deficiency, plants induce an array of physiological and morphological responses, termed phosphate starvation response (PSR), thereby increasing Pi acquisition and use efficiency. However, the mechanisms by which plants adapt to Pi deficiency remain to be elucidated. Here, we report that deposition of a β-1,3-glucan polymer called callose is induced in Arabidopsis thaliana root hairs under Pi deficiency, in a manner independent of PSR-regulating PHR1/PHL1 transcription factors and LPR1/LPR2 ferroxidases. Genetic studies revealed PMR4 (GSL5) callose synthase being required for the callose deposition in Pi-depleted root hairs. Loss of PMR4 also reduces Pi acquisition in shoots and plant growth under low Pi conditions. The defects are not recovered by simultaneous disruption of SID2, mediating defense-associated salicylic acid (SA) biosynthesis, excluding SA defense activation from the cause of the observed pmr4 phenotypes. Grafting experiments and characterization of plants expressing PMR4 specifically in root hair cells suggest that a PMR4 pool in the cell type contributes to shoot growth under Pi deficiency. Our findings thus suggest an important role for PMR4 in plant adaptation to Pi deficiency.
期刊介绍:
Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community.
Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.