Biomimetic mineralization of positively charged silica nanoparticles templated by thermoresponsive protein micelles: applications to electrostatic assembly of hierarchical and composite superstructures.

IF 2.9 3区 化学 Q3 CHEMISTRY, PHYSICAL
Soft Matter Pub Date : 2024-11-11 DOI:10.1039/d4sm00907j
Nada Y Naser, William C Wixson, Helen Larson, Brandi M Cossairt, Lilo D Pozzo, François Baneyx
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引用次数: 0

Abstract

High information content building blocks offer a path toward the construction of precision materials by supporting the organization and reconfiguration of organic and inorganic components through engineered functions. Here, we combine thermoresponsiveness with biomimetic mineralization by fusing the Car9 silica-binding dodecapeptide to the C-terminus of the (VPGVG)54 elastin-like polypeptide (ELP). Using small angle X-ray scattering, we show that the short Car9 cationic block is sufficient to promote the conversion of disordered unimers into 30 nm micelles comprising about 150 proteins, 5 °C above the transition temperature of the ELP. While both species catalyze self-limiting silica precipitation, micelles template the mineralization of highly monodisperse (62 nm) nanoparticles, while unimers yield larger polydisperse species. Strikingly, and unlike traditional synthetic silica, these particles exhibit a positive surface charge, likely due to cationic Car9 sidechains projecting from their surface. Capitalizing on the high monodispersity and positive charge of the micelle-templated products, we use smaller silica and gold particles bearing a native negative charge to create a variety of superstructures via electrostatic co-assembly. This simple biomimetic route to positively charged silica eliminates the need for multiple precursors or surface modifications and enables the rapid creation of single-material and composite architectures in which components of different sizes or compositions are well dispersed and integrated.

以热膨胀蛋白胶束为模板的带正电的二氧化硅纳米粒子的仿生矿化:应用于分层和复合超结构的静电组装。
高信息含量构建模块通过工程功能支持有机和无机成分的组织和重组,为构建精密材料提供了一条途径。在这里,我们通过将 Car9 二氧化硅结合十二肽与 (VPGVG)54 弹性蛋白样多肽 (ELP) 的 C 端融合,将热响应性与仿生矿化结合起来。利用小角 X 射线散射,我们发现短 Car9 阳离子嵌段足以促进无序单聚体转化为 30 nm 的胶束,其中包含约 150 个蛋白质,比 ELP 的转变温度高 5 °C。虽然这两种物质都能催化自限性二氧化硅沉淀,但胶束是高度单分散(62 nm)纳米颗粒矿化的模板,而单聚物则产生较大的多分散物质。引人注目的是,与传统的合成二氧化硅不同,这些微粒表面带有正电荷,这可能是由于阳离子 Car9 侧链从其表面伸出所致。利用胶束模板产品的高单分散性和正电荷,我们使用带有原生负电荷的较小二氧化硅和金颗粒,通过静电共组装创造出各种超结构。这种获得带正电的二氧化硅的简单仿生途径无需使用多种前体或进行表面改性,因此能快速制造出单一材料和复合材料结构,其中不同尺寸或成分的组分能很好地分散和整合在一起。
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来源期刊
Soft Matter
Soft Matter 工程技术-材料科学:综合
CiteScore
6.00
自引率
5.90%
发文量
891
审稿时长
1.9 months
期刊介绍: Soft Matter is an international journal published by the Royal Society of Chemistry using Engineering-Materials Science: A Synthesis as its research focus. It publishes original research articles, review articles, and synthesis articles related to this field, reporting the latest discoveries in the relevant theoretical, practical, and applied disciplines in a timely manner, and aims to promote the rapid exchange of scientific information in this subject area. The journal is an open access journal. The journal is an open access journal and has not been placed on the alert list in the last three years.
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