Factors impacting tumor displacement artifact and pseudo-lymphovascular invasion in testicular germ cell tumors.

IF 2.3 4区医学 Q2 PATHOLOGY

American journal of clinical pathology Pub Date : 2024-11-09 DOI:10.1093/ajcp/aqae147

Reem O Ahmed, Debra L Zynger

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引用次数: 0

Abstract

Objectives: Testicular germ cell tumors are susceptible to tumor displacement artifact (TDA), which produces pseudo-lymphovascular invasion (LVI) and confounds the identification of true LVI. Our study aimed to evaluate tumor displacement artifact and pseudo-LVI in testicular germ cell tumors and determine if prolonged fixation improves histological quality.

Methods: A retrospective search identified 121 orchiectomies with slides that were reviewed to assess TDA and pseudo-LVI.

Results: Seminoma had more TDA (68% vs 45%, P = .01) and pseudo-LVI (53% vs 19%, P < .001) than mixed germ cell tumor. Seminoma and mixed germ cell tumor with TDA and pseudo-LVI were larger than those without. Mixed germ cell tumor with ≥50% composition of seminoma had a higher rate of TDA (89% vs 38%, P = .008) and pseudo-LVI (44% vs 15%, P = .06) than those with less. TDA was reduced in seminoma with >1 night fixation compared to no fixation (50% vs 82%, P = .046), with a similar trend in mixed germ cell tumor (31% vs 60%, P = .15). A trend in reduction of pseudo-LVI was seen with >1 night fixation compared to no fixation in seminoma (64% vs 39%, P = .12).

Conclusions: Seminomas and larger germ cell tumors were more prone to TDA and pseudo-LVI. Prolonged formalin fixation improved histological quality in testicular germ cell tumors. Based on these data, we recommend fixation for at least 2 nights before sectioning orchiectomy specimens, particularly for larger tumors.

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影响睾丸生殖细胞瘤肿瘤移位假象和假淋巴管侵犯的因素

目的：睾丸生殖细胞肿瘤易受肿瘤移位伪影（TDA）的影响，TDA会产生假性淋巴管侵犯（LVI），混淆真性LVI的鉴定。我们的研究旨在评估睾丸生殖细胞肿瘤中的肿瘤移位伪影和假性LVI，并确定延长固定时间是否能提高组织学质量：方法：通过回顾性检索确定了121例睾丸切除术，并对切片进行了审查，以评估TDA和假性LVI：结果：与混合生殖细胞瘤相比，精原细胞瘤有更多的TDA（68% vs 45%，P = .01）和假性LVI（53% vs 19%，P < .001）。有TDA和假性LVI的精原细胞瘤和混合生殖细胞瘤比没有TDA和假性LVI的要大。精原细胞瘤成分≥50%的混合生殖细胞瘤的TDA（89% vs 38%，P = .008）和假性LVI（44% vs 15%，P = .06）率高于成分较少的混合生殖细胞瘤。精原细胞瘤固定时间大于 1 晚的 TDA 比未固定的要低（50% vs 82%，P = .046），混合生殖细胞瘤也有类似趋势（31% vs 60%，P = .15）。在精原细胞瘤中，与不固定相比，固定>1晚后假性LVI有下降趋势（64% vs 39%，P = .12）：结论：精原细胞瘤和较大的生殖细胞瘤更容易发生TDA和假性LVI。结论：精原细胞瘤和较大的生殖细胞瘤更容易发生 TDA 和假性 LVI，长期福尔马林固定可改善睾丸生殖细胞瘤的组织学质量。基于这些数据，我们建议在睾丸切除术标本切片前至少固定两晚，尤其是较大的肿瘤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

American journal of clinical pathology 医学-病理学

CiteScore

7.70

自引率

2.90%

发文量

367

审稿时长

3-6 weeks

期刊介绍： The American Journal of Clinical Pathology (AJCP) is the official journal of the American Society for Clinical Pathology and the Academy of Clinical Laboratory Physicians and Scientists. It is a leading international journal for publication of articles concerning novel anatomic pathology and laboratory medicine observations on human disease. AJCP emphasizes articles that focus on the application of evolving technologies for the diagnosis and characterization of diseases and conditions, as well as those that have a direct link toward improving patient care.