Ultrasonication-mediated synthesis of diblock polymer-based nanoparticles for advanced drug delivery systems: Insights and optimization

IF 8.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry Pub Date : 2024-10-29 DOI:10.1016/j.ultsonch.2024.107137

Nagaraja Sreeharsha , Srikruthi Kunigal Sridhar , Asha Bhuvanahalli Rangappa , Prakash Goudanavar , Purushotham Karadigere Nagaraju , Nimbagal Raghavendra Naveen , Predeepkumar Narayanappa Shiroorkar , Afzal Haq Asif , Girish Meravanige , Krishna Swaroop Duddi Sreehari

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Abstract

This study presents the synthesis and optimization of Methylene polyethyl glycol −Polystyrene (mPEG-PS) Diblock (DIP) copolymer-based solid lipid nanoparticles (SLNs) using ultrasonication for advanced drug delivery systems targeting the human immunodeficiency virus (HIV-1). The mPEG-PS block copolymer was synthesized by ring opening polymerization mechanism under nitrogen atmosphere for 24hrs and characterized using Fourier Transform Infrared Spectroscopy (FTIR) spectroscopy and NMR, confirming the formation of DIP polymers. Optimization of SLNs formulation was achieved through a systematic approach, utilizing response surface methodology, optimal conditions for SLNs synthesis were determined, resulting in nanoparticles with a particle size of 198 nm and an entrapment efficiency of 67.42 %. Cell viability assays, quantitative PCR for viral DNA analysis, caspase-3 enzyme assays, and quantitative uptake studies using High Performance Liquid Chromatography (HPLC) provided quantitative insights into the efficacy and biocompatibility of the synthesized nanoparticles. The experimental data demonstrate that nanoparticle treatments significantly influence cellular responses, providing valuable insights into their therapeutic potential and underlying mechanisms. By employing precise experimental methods alongside rigorous analytical techniques, this study enhances our understanding of nanoparticle-based drug delivery systems, particularly in the context of HIV treatment. These findings pave the way for optimizing therapeutic strategies to improve patient outcomes.

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超声介导合成二嵌段聚合物纳米颗粒，用于先进的给药系统：见解与优化。

本研究介绍了亚甲基聚乙二醇-聚苯乙烯（mPEG-PS）二嵌段（DIP）共聚物的合成和优化，该共聚物基于超声波，可用于靶向人类免疫缺陷病毒（HIV-1）的先进药物递送系统。mPEG-PS 嵌段共聚物是在氮气环境下通过开环聚合机理经 24 小时合成的，傅立叶变换红外光谱（FTIR）和核磁共振对其进行了表征，证实了 DIP 聚合物的形成。通过系统化方法优化了 SLNs 的配方，利用响应面方法确定了 SLNs 合成的最佳条件，最终得到粒径为 198 nm 的纳米颗粒，包埋效率为 67.42%。通过细胞活力测定、定量 PCR 病毒 DNA 分析、Caspase-3 酶测定以及使用高效液相色谱法（HPLC）进行的定量吸收研究，可以定量了解合成纳米粒子的功效和生物相容性。实验数据表明，纳米粒子处理对细胞反应有显著影响，为了解其治疗潜力和潜在机制提供了宝贵的信息。通过采用精确的实验方法和严格的分析技术，这项研究加深了我们对基于纳米粒子的给药系统的了解，尤其是在艾滋病治疗方面。这些发现为优化治疗策略、改善患者预后铺平了道路。

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来源期刊

Ultrasonics Sonochemistry 化学-化学综合

CiteScore

15.80

自引率

11.90%

发文量

361

审稿时长

59 days

期刊介绍： Ultrasonics Sonochemistry stands as a premier international journal dedicated to the publication of high-quality research articles primarily focusing on chemical reactions and reactors induced by ultrasonic waves, known as sonochemistry. Beyond chemical reactions, the journal also welcomes contributions related to cavitation-induced events and processing, including sonoluminescence, and the transformation of materials on chemical, physical, and biological levels. Since its inception in 1994, Ultrasonics Sonochemistry has consistently maintained a top ranking in the "Acoustics" category, reflecting its esteemed reputation in the field. The journal publishes exceptional papers covering various areas of ultrasonics and sonochemistry. Its contributions are highly regarded by both academia and industry stakeholders, demonstrating its relevance and impact in advancing research and innovation.