An innovative fluorescent probe for monitoring of ONOO^- in multiple liver-injury models.

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2025-02-01 Epub Date: 2024-11-08 DOI:10.1016/j.talanta.2024.127194

Cailing Fan, Kaifu Ma, Weijie Chi, Yongwei LiMeng, Qinxi Dong, Yanan Gao, Chaokun Zeng, Wenshu Meng, Wei Shu, Chaoyuan Zeng

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引用次数: 0

Abstract

The liver plays a pivotal role in numerous critical physiological processes, functioning as the body's metabolic and detoxification center. Chronic liver disease can precipitate more severe health complications. The onset and progression of liver disease are often characterized by abnormal concentrations of ONOO^-, a highly reactive species whose direct capture and detection in physiological environments pose significant challenges. This work presents an innovative fluorescent probe NAP-ONOO derived from 1,8-naphthalimide, specifically engineered to dynamically monitor fluctuations of ONOO^- levels during liver injury. Due to its high biocompatibility, NAP-ONOO enabled to observe varying degrees of ONOO^- up-regulation across models of liver inflammatory injury, alcohol-induced damage, and drug-induced hepatotoxicity in cellular systems as well as in zebrafish and mice models. These findings highlight the potential of NAP-ONOO for identifying and detecting the liver injury biomarker ONOO^-. Furthermore, NAP-ONOO serves as potent tool for the identification of liver injuries, drug screening, and cellular imaging analyses, thereby promising avenues for future research endeavors.

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用于监测多种肝损伤模型中 ONOO- 的创新型荧光探针。

肝脏在许多关键的生理过程中发挥着举足轻重的作用，是人体的代谢和解毒中心。慢性肝病会引发更严重的健康并发症。ONOO-是一种高活性物质，其在生理环境中的直接捕获和检测面临巨大挑战。本研究提出了一种创新的荧光探针 NAP-ONOO，它源自 1,8-萘二甲酰亚胺，专门用于动态监测肝损伤过程中 ONOO- 水平的波动。由于 NAP-ONOO 具有很高的生物相容性，因此能在细胞系统以及斑马鱼和小鼠模型中观察到不同程度的 ONOO- 上调，包括肝脏炎症损伤、酒精诱导的损伤和药物诱导的肝毒性。这些发现凸显了 NAP-ONOO 在识别和检测肝损伤生物标志物 ONOO- 方面的潜力。此外，NAP-ONOO 还是鉴定肝损伤、药物筛选和细胞成像分析的有效工具，为未来的研究工作提供了广阔的前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.