Transcriptomic analysis reveals decreased expression of detoxification genes in Caenorhabditis elegans exposed to mepanipyrim and cyprodinil: Implications for multigenerational exposure effects

IF 6.2 2区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Ecotoxicology and Environmental Safety Pub Date : 2024-11-15 DOI:10.1016/j.ecoenv.2024.117265

Tingting Gai , Lianfeng Zhang , Liangwen Chen , Yun Wang , Xiaonan Sun , Yuhao Yang , Wenjing Cao , Yanyan Chen

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Abstract

The broad-spectrum fungicides mepanipyrim (Mep) and cyprodinil (Cyp) have been reported to be used worldwide to control gray mold of fruit crops. Consequently, they are often detected in the water and food items. However, the impacts and potential mechanisms of these two pesticides on environmental organisms remain unclear. Utilizing Caenorhabditis elegans (C. elegans) as the model, the toxic effects and mechanisms were analyzed after Mep and Cyp exposure over four generations (P₀-F₃). The results showed that Mep and Cyp at concentrations over 0.1 µg/L affected the motility behavior, while 1.0 µg/L dosage and above inhibited the growth of C. elegans. In addition, multigenerational exposure could significantly impair both locomotion and development of C. elegans even at 0.01 µg/L dosage. Notably, constant exposure resulted in the function abnormalities of cholinergic, dopaminergic and GABAergic neurons, as well as the accumulation of ROS. Further transcriptome analysis showed reduced expression of 14 genes and 26 genes in xenobiotic metabolic pathway following Mep and Cyp exposure, respectively. The buildup of Mep/Cyp and ROS might have led to nerve impairment and behavioral abnormality. These findings enhanced understanding of the toxic effects of Mep and Cyp and provided insights into their eco-toxicological evaluation.

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转录组分析显示，暴露于氨苯嘧啶和环丙嘧草酯的秀丽隐杆线虫体内的解毒基因表达量减少：对多代接触效应的影响。

据报道，世界各地都在使用广谱杀菌剂嘧霉胺（Mep）和嘧菌环胺（Cyp）来控制水果作物的灰霉病。因此，水和食品中经常检测到这两种杀菌剂。然而，这两种杀虫剂对环境生物的影响和潜在机制仍不清楚。本研究以 elegans（秀丽隐杆线虫）为模型，分析了四代（P0-F3）暴露于 Mep 和 Cyp 后的毒性效应和机制。结果表明，浓度超过 0.1 µg/L 的 Mep 和 Cyp 会影响 elegans 的运动行为，而 1.0 µg/L 及以上的剂量会抑制 elegans 的生长。此外，即使剂量为 0.01 µg/L，多代接触也会严重影响秀丽隐杆线虫的运动和发育。值得注意的是，持续暴露会导致胆碱能、多巴胺能和 GABA 能神经元的功能异常以及 ROS 的积累。进一步的转录组分析表明，暴露于 Mep 和 Cyp 后，分别有 14 个基因和 26 个基因在异生物代谢途径中表达减少。Mep/Cyp和ROS的积累可能会导致神经损伤和行为异常。这些发现加深了人们对 Mep 和 Cyp 毒性效应的理解，并为生态毒理学评估提供了见解。

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来源期刊

Ecotoxicology and Environmental Safety 环境科学-毒理学

CiteScore

12.10

自引率

5.90%

发文量

1234

审稿时长

88 days

期刊介绍： Ecotoxicology and Environmental Safety is a multi-disciplinary journal that focuses on understanding the exposure and effects of environmental contamination on organisms including human health. The scope of the journal covers three main themes. The topics within these themes, indicated below, include (but are not limited to) the following: Ecotoxicology、Environmental Chemistry、Environmental Safety etc.