Overexpression of PtNRPS1 enhances diatom-mediated bioremediation of salicylate pollution

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology Pub Date : 2024-11-07 DOI:10.1016/j.biortech.2024.131782

Dong-Sheng Zhao , Yu-Ting Chen , Jia-Min Xu , Xiao-Li Liu , Yi-Cheng Xu , Peng Cao , Junliang Li , Shengqin Wang , Nan Li , Yong Li , Shu-Ming Li , Xiufeng Yan , Qiuying Pang , Hui-Xi Zou

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Abstract

The accumulation of the emerging pollutant salicylic acid (SA) in the environment has gained much attention. In this study, overexpression of the non-ribosomal peptide synthase (NRPS) gene, PtNRPS1 in the marine diatom Phaeodactylum tricornutum (PtNRPS1-OE) increased resistance to SA pollutants. It was assumed that the enhanced tolerance was due to the high binding affinity between recombinant PtNRPS1 (rNRPS1) and SA pollutants. Moreover, tandem mass spectrometry analysis determined the amino acids that participated in the covalently binding of SA. The removal efficiency of SA pollutants by PtNRPS1-OE cells was found to be markedly elevated. The mechanism underlying the removal of SA and 5-substituted SA (5-sSA) was proposed, following the co-localization analysis of rNRPS1 and SA. The purpose of this study was not about using PtNRPS1 as an enzyme to catalyze the synthesis of metabolite. Rather, it explored the possibility of using PtNRPS1 to remove pollutants, which further improves practical feasibility of microalgae-mediated bioremediation.

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过表达 PtNRPS1 可增强硅藻介导的水杨酸盐污染生物修复。

新出现的污染物水杨酸（SA）在环境中的积累已引起广泛关注。在这项研究中，海洋硅藻 Phaeodactylum tricornutum（PtNRPS1-OE）过表达非核糖体肽合成酶（NRPS）基因 PtNRPS1 增加了对 SA 污染物的耐受性。据推测，耐受性的增强是由于重组 PtNRPS1（rNRPS1）与 SA 污染物之间的高结合亲和力。此外，串联质谱分析确定了参与与 SA 共价结合的氨基酸。研究发现，PtNRPS1-OE 细胞对 SA 污染物的去除率明显提高。通过对 rNRPS1 和 SA 的共定位分析，提出了去除 SA 和 5-取代的 SA（5-sSA）的机制。本研究的目的不是利用 PtNRPS1 作为催化合成代谢物的酶。而是探索利用 PtNRPS1 去除污染物的可能性，从而进一步提高微藻介导的生物修复的实际可行性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioresource Technology 工程技术-能源与燃料

CiteScore

20.80

自引率

19.30%

发文量

2013

审稿时长

12 days

期刊介绍： Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies. Topics include: • Biofuels: liquid and gaseous biofuels production, modeling and economics • Bioprocesses and bioproducts: biocatalysis and fermentations • Biomass and feedstocks utilization: bioconversion of agro-industrial residues • Environmental protection: biological waste treatment • Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.