Carbon fate and potential carbon metabolism effects during in-situ cyanobacterial inhibition by artemisinin sustained-release algaecides

Abstract

Carbon pools and microbial carbon metabolism can be significantly altered due to the diverse organic matter properties and low pH characteristics of artemisinin sustained-release algaecides (ASAs). These effects have still not been systematically studied, leading to uncertainty in the application of ASAs for cyanobacterial management in natural waters. This study assessed the effects of ASAs on carbon fate, carbon metabolism and potential ecological impacts during in-situ cyanobacterial inhibition. In the initial phase of ASAs-induced cyanobacterial inhibition (2-10 days), the carbon pool underwent significant changes due to the increased proportion of C4 plant-derived organic matter (97%), humification level (FI =0.9-1.5), and inorganic carbon concentration (TIC = 80-110 mg/L). The cyanobacterial apoptosis triggered by ASAs produced particulate organic carbon, which provided a bioavailable carbon source for bacterial metabolism. ASAs enhanced the connection between bacteria and the carbon pool, as well as their carbon metabolism capabilities, by increasing the relative abundance of Proteobacteria (33.1%-37.3%), bacterial diversity, the proportion of Alphaproteobacteria and Betaproteobacteria (19.3%-29.5%) involved in carbon metabolism, and the complexity of the environment-bacteria co-occurrence network. These effects contributed to maintaining long-term ecosystem stability and resistance to cyanobacterial proliferation. Our findings elucidate the influence of bacterial carbon metabolism by ASAs as one of an important mechanism for achieving cyanobacterial inhibition in natural water, and emphasize the important role of bacteria in maintaining ecological stability during in situ cyanobacterial management.