Proteomic Insights Into Gingival Crevicular Extracellular Vesicles in Periodontitis and Gestational Diabetes: An Exploratory Study

IF 5.8 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Clinical Periodontology Pub Date : 2024-11-13 DOI:10.1111/jcpe.14083

María Luisa Mizgier, Gino Nardocci, Valeria Ramírez, María José Bendek, Marcela Hernández, Carolina Rojas, David Herrera, Alpdogan Kantarci, Matthew W. Kemp, Sebastián E. Illanes, Alejandra Chaparro

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Abstract

AimTo characterize the gingival crevicular fluid (GCF) and plasma extracellular vesicles (EVs) and explore their proteomic cargo in healthy pregnant women compared to those with gestational diabetes mellitus (GDM) and periodontitis.MethodsOne‐hundred and four pregnant women were recruited at 24–30 gestation weeks. GDM was diagnosed by an oral glucose tolerance test. GCF and plasma samples were obtained to isolate EVs and characterized by nanoparticle tracking, immunoassays, electron microscopy and mass spectrometry.ResultsOf the recruits,17.3% women were healthy, 50% had periodontitis and 32.7% had both GDM and periodontitis. Probing depth, clinical attachment loss and bleeding on probing were more severe in GDM and periodontitis pregnancies (p < 0.0001). Additionally, this group showed an increase concentration of total, small and large GCF‐EVs (p = 0.0015, p = 0.0011 and p = 0.0008, respectively), with decreased expression of CD9, CD81 and CD81/CD63 ratio (p = 0.0461, p = 0.0164 and p = 0.0005, respectively). No differences were observed in plasmatic EVs concentration or markers expression. Proteomic analysis of GCF‐EVs showed peptides of both host and bacterial origin. Gene ontology analysis revealed that proteins of GCF‐EVs participate in immune inflammatory responses, glucose metabolism and insulin response mechanisms.ConclusionGCF‐EVs were increased in both GDM and periodontitis, and their proteomic cargo suggest their involvement in immune inflammatory response, glucose metabolism and insulin pathways during pregnancy.

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牙周炎和妊娠糖尿病患者牙龈细胞外泡的蛋白质组学研究：一项探索性研究

目的研究健康孕妇与妊娠期糖尿病（GDM）和牙周炎孕妇的牙龈缝隙液（GCF）和血浆细胞外囊泡（EVs）的特征，并探索其蛋白质组货物。通过口服葡萄糖耐量试验诊断出 GDM。采集 GCF 和血浆样本以分离 EVs，并通过纳米粒子追踪、免疫测定、电子显微镜和质谱分析对 EVs 进行表征。在 GDM 和牙周炎孕妇中，探诊深度、临床附着丧失和探诊出血更为严重（p < 0.0001）。此外，这组孕妇的总GCF-EVs、小GCF-EVs和大GCF-EVs浓度增加（分别为p = 0.0015、p = 0.0011和p = 0.0008），CD9、CD81和CD81/CD63比值表达降低（分别为p = 0.0461、p = 0.0164和p = 0.0005）。在血浆 EVs 浓度或标志物表达方面未观察到差异。GCF-EVs的蛋白质组分析显示，肽既有宿主来源的，也有细菌来源的。结论GCF-EVs在GDM和牙周炎中均有增加，其蛋白质组货物表明它们参与了妊娠期间的免疫炎症反应、葡萄糖代谢和胰岛素通路。

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来源期刊

Journal of Clinical Periodontology 医学-牙科与口腔外科

CiteScore

13.30

自引率

10.40%

发文量

175

审稿时长

3-8 weeks

期刊介绍： Journal of Clinical Periodontology was founded by the British, Dutch, French, German, Scandinavian, and Swiss Societies of Periodontology. The aim of the Journal of Clinical Periodontology is to provide the platform for exchange of scientific and clinical progress in the field of Periodontology and allied disciplines, and to do so at the highest possible level. The Journal also aims to facilitate the application of new scientific knowledge to the daily practice of the concerned disciplines and addresses both practicing clinicians and academics. The Journal is the official publication of the European Federation of Periodontology but wishes to retain its international scope. The Journal publishes original contributions of high scientific merit in the fields of periodontology and implant dentistry. Its scope encompasses the physiology and pathology of the periodontium, the tissue integration of dental implants, the biology and the modulation of periodontal and alveolar bone healing and regeneration, diagnosis, epidemiology, prevention and therapy of periodontal disease, the clinical aspects of tooth replacement with dental implants, and the comprehensive rehabilitation of the periodontal patient. Review articles by experts on new developments in basic and applied periodontal science and associated dental disciplines, advances in periodontal or implant techniques and procedures, and case reports which illustrate important new information are also welcome.