MoS2-mediated gap-mode surface plasmon enhancement: Construction of SPR biosensor for direct detection of LECT2

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2024-11-14 DOI:10.1016/j.snb.2024.136938

Yangyang Zhou , Hezhen Liu , Weiwen Zhang , Jingyao Wu , Kwangnak Koh , Hongxia Chen

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引用次数: 0

Abstract

Serum levels of leukocyte cell-derived chemotaxin-2 (LECT2) have emerged as promising diagnostic biomarker for liver fibrosis. Targeting the LECT2/Tie1 signaling pathway holds potential for innovative treatment approaches to this condition. Despite this, the development of highly sensitive assays for serum LECT2 remains limited. Surface plasmon resonance (SPR), a label-free technique for detecting biomolecular interactions via refractive index (RI) changes, has demonstrated notable sensitivity for biomarker assays, though improving its sensitivity for direct LECT2 detection faces certain challenges. In this study, we present an innovative enhanced SPR biosensor designed to overcome current limitations in LECT2 detection. By incorporating molybdenum disulfide (MoS₂) nanosheets as nano-spacer between the gold film and cyclodextrin functionalized-gold nanoparticles (CD-AuNPs), this gap-mode design significantly enhances the electric field intensity near the gold film surface, which effectively improves the detection sensitivity of the RI changes. Compared to traditional gold-film-only SPR sensors, our sensor achieves a remarkable sensitivity increase from 73.1 to 133.3 deg/RIU, with a commendable quality factor and detection accuracy. By further modifying the specific peptide of the terminal label phenylalanine (TFF) through the host-guest recognition effect of the CD hydrophobic cavity, we achieved a broad linear range and a low limit of detection (LOD = 0.6206 ng/mL) for the detection of the liver fibrosis marker LECT2. Our proposed enhanced SPR sensing platform demonstrates significant potential for clinical applications in LECT2 detection.

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MoS2- 介导的间隙模式表面等离子体增强：构建用于直接检测 LECT2 的 SPR 生物传感器

血清中的白细胞源性趋化因子-2（LECT2）水平已成为肝纤维化的有希望的诊断生物标志物。以 LECT2/Tie1 信号通路为靶点，有可能成为治疗肝纤维化的创新方法。尽管如此，针对血清 LECT2 的高灵敏度检测方法的开发仍然有限。表面等离子体共振（SPR）是一种通过折射率（RI）变化检测生物分子相互作用的无标记技术，已在生物标记物检测中显示出显著的灵敏度，但提高其灵敏度以直接检测 LECT2 还面临着一定的挑战。在本研究中，我们提出了一种创新的增强型 SPR 生物传感器，旨在克服目前 LECT2 检测中的局限性。通过在金膜和环糊精功能化金纳米粒子（CD-AuNPs）之间加入二硫化钼（MoS2）纳米片作为纳米垫片，这种间隙模式设计显著增强了金膜表面附近的电场强度，从而有效提高了 RI 变化的检测灵敏度。与传统的纯金膜 SPR 传感器相比，我们的传感器实现了灵敏度从 73.1 度/RIU 到 133.3 度/RIU 的显著提高，其品质因数和检测精度也值得称赞。通过 CD 疏水腔的主客识别效应进一步修饰末端标记苯丙氨酸（TFF）的特异性肽，我们实现了检测肝纤维化标志物 LECT2 的宽线性范围和低检测限（LOD = 0.6206 ng/mL）。我们提出的增强型 SPR 传感平台在 LECT2 检测的临床应用中展示了巨大的潜力。

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.