Epidemiologic and genomic characterization of an outbreak of Rift Valley fever among humans and dairy cattle in northern Tanzania

Deng B Madut, Matthew P Rubach, Kathryn J Allan, Kate M Thomas, William A de Glanville, Jo E B Halliday, Cristina Costales, Manuela Carugati, Robert J Rolfe, John P Bonnewell, Michael J Maze, Alex R Mremi, Patrick T Amsi, Nathaniel H Kalengo, Furaha Lyamuya, Grace D Kinabo, Ronald Mbwasi, Kajiru G Kilonzo, Venance P Maro, Blandina T Mmbaga, Bingileki Lwezaula, Calvin Mosha, Annette Marandu, Tito J Kibona, Feng Zhu, Tanu Chawla, Wan Ni Chia, Danielle E Anderson, Lin-Fa Wang, Jie Liu, Eric R Houpt, Roosecelis B Martines, Sherif R Zaki, Austin Leach, Aridth Gibbons, Cheng-Feng Chiang, Ketan Patel, John D Klena, Sarah Cleaveland, John A Crump
{"title":"Epidemiologic and genomic characterization of an outbreak of Rift Valley fever among humans and dairy cattle in northern Tanzania","authors":"Deng B Madut, Matthew P Rubach, Kathryn J Allan, Kate M Thomas, William A de Glanville, Jo E B Halliday, Cristina Costales, Manuela Carugati, Robert J Rolfe, John P Bonnewell, Michael J Maze, Alex R Mremi, Patrick T Amsi, Nathaniel H Kalengo, Furaha Lyamuya, Grace D Kinabo, Ronald Mbwasi, Kajiru G Kilonzo, Venance P Maro, Blandina T Mmbaga, Bingileki Lwezaula, Calvin Mosha, Annette Marandu, Tito J Kibona, Feng Zhu, Tanu Chawla, Wan Ni Chia, Danielle E Anderson, Lin-Fa Wang, Jie Liu, Eric R Houpt, Roosecelis B Martines, Sherif R Zaki, Austin Leach, Aridth Gibbons, Cheng-Feng Chiang, Ketan Patel, John D Klena, Sarah Cleaveland, John A Crump","doi":"10.1093/infdis/jiae562","DOIUrl":null,"url":null,"abstract":"Background A peri-urban outbreak of Rift Valley fever virus (RVFV) among dairy cattle from May through August 2018 in northern Tanzania was detected through testing samples from prospective livestock abortion surveillance. We sought to identify concurrent human infections, their phylogeny, and epidemiologic characteristics in a cohort of febrile patients enrolled from 2016-2019 at hospitals serving the epizootic area. Methods From September 2016 through May 2019, we conducted a prospective cohort study that enrolled febrile patients hospitalized at two hospitals in Moshi, Tanzania. Archived serum, plasma, or whole blood samples were retrospectively tested for RVFV by PCR. Human samples positive for RVFV were sequenced and compared to RVFV sequences obtained from cattle through a prospective livestock abortion study. Phylogenetic analysis was performed on complete RVFV genomes. Results Among 656 human participants, we detected RVFV RNA in four (0.6%), including one death with hepatic necrosis and other end-organ damage at autopsy. Humans infected with RVFV were enrolled from June through August 2018, and all resided in or near urban areas. Phylogenetic analysis of human and cattle RVFV sequences demonstrated that most clustered to lineage B, a lineage previously described in East Africa. A lineage E strain clustering with lineages in Angola was also identified in cattle. Conclusion We provide evidence that an apparently small RVFV outbreak among dairy cattle in northern Tanzania was associated with concurrent severe and fatal infections among humans. Our findings highlight the unidentified scale and diversity of inter-epizootic RVFV transmission, including near and within an urban area.","PeriodicalId":501010,"journal":{"name":"The Journal of Infectious Diseases","volume":"20 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Journal of Infectious Diseases","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1093/infdis/jiae562","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Background A peri-urban outbreak of Rift Valley fever virus (RVFV) among dairy cattle from May through August 2018 in northern Tanzania was detected through testing samples from prospective livestock abortion surveillance. We sought to identify concurrent human infections, their phylogeny, and epidemiologic characteristics in a cohort of febrile patients enrolled from 2016-2019 at hospitals serving the epizootic area. Methods From September 2016 through May 2019, we conducted a prospective cohort study that enrolled febrile patients hospitalized at two hospitals in Moshi, Tanzania. Archived serum, plasma, or whole blood samples were retrospectively tested for RVFV by PCR. Human samples positive for RVFV were sequenced and compared to RVFV sequences obtained from cattle through a prospective livestock abortion study. Phylogenetic analysis was performed on complete RVFV genomes. Results Among 656 human participants, we detected RVFV RNA in four (0.6%), including one death with hepatic necrosis and other end-organ damage at autopsy. Humans infected with RVFV were enrolled from June through August 2018, and all resided in or near urban areas. Phylogenetic analysis of human and cattle RVFV sequences demonstrated that most clustered to lineage B, a lineage previously described in East Africa. A lineage E strain clustering with lineages in Angola was also identified in cattle. Conclusion We provide evidence that an apparently small RVFV outbreak among dairy cattle in northern Tanzania was associated with concurrent severe and fatal infections among humans. Our findings highlight the unidentified scale and diversity of inter-epizootic RVFV transmission, including near and within an urban area.
坦桑尼亚北部人类和奶牛裂谷热疫情的流行病学和基因组特征分析
背景通过对前瞻性牲畜流产监测样本的检测,发现 2018 年 5 月至 8 月坦桑尼亚北部城市周边地区爆发了奶牛裂谷热病毒(RVFV)疫情。我们试图在2016-2019年期间服务于疫区医院的发热患者队列中识别并发的人类感染、其系统发育和流行病学特征。方法 从 2016 年 9 月到 2019 年 5 月,我们开展了一项前瞻性队列研究,招募了在坦桑尼亚莫希市两家医院住院的发热患者。通过 PCR 对存档血清、血浆或全血样本进行了 RVFV 回顾性检测。对 RVFV 阳性的人体样本进行了测序,并与通过前瞻性牲畜流产研究从牛身上获得的 RVFV 序列进行了比较。对完整的 RVFV 基因组进行了系统发育分析。结果 在 656 名人类参与者中,我们在 4 人(0.6%)体内检测到了 RVFV RNA,其中 1 人在尸检时因肝坏死和其他内脏器官损伤而死亡。感染 RVFV 的人类是在 2018 年 6 月至 8 月期间加入的,他们都居住在城市地区或城市附近。人和牛 RVFV 序列的系统进化分析表明,大部分聚类为 B 系,这是以前在东非描述过的一个系。在牛身上还发现了一个与安哥拉各系相近的 E 系菌株。结论 我们提供的证据表明,在坦桑尼亚北部奶牛中爆发的看似小规模的 RVFV 疫情与同时发生的严重和致命的人类感染有关。我们的研究结果突显了 RVFV 疫情间传播的未知规模和多样性,包括在城市附近和城市内部的传播。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信