Identification of the Active EPA/AA-Binding Ether-Type Phosphatidylcholine Derived from the Starfish Patiria pectinifera for C2C12 Myotube Growth

IF 4.3 3区材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC

ACS Applied Electronic Materials Pub Date : 2024-10-30 DOI:10.1021/acsomega.4c0786510.1021/acsomega.4c07865

Aoi Fukushima, Kyosuke Imamura, Naoki Takatani, Masashi Hosokawa and Fumiaki Beppu*,

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引用次数: 0

Abstract

Concerns about nutritional approaches for promoting skeletal muscle mass and function have increased. This study assessed the effects of starfish-derived glycerophospholipids (PLs) (SPL), characterized by unique ether-linked subclasses, alkylacyl (Alk)- and alkenylacyl (Pls)-PL, on skeletal muscle function, focusing on myotube formation in C2C12 myoblasts. SPL was prepared via chloroform/methanol extraction from Patiria pectinifera, followed by silica gel chromatography fractionation. Myoblasts were induced to differentiate with or without SPL treatment. On day 7 of differentiation, 50 μg/mL of SPL treatment increased myotube diameter. The phosphatidylcholine (PC) fraction (SPC) also enhanced myotube growth at 30 μg/mL. LC–MS/MS analysis indicated the most abundant PC molecular species in SPC were Alk- and Pls-PC with eicosapentaenoic acid and arachidonic acid. Treatment with 1-O-hexadecyl-2-arachidonoyl-PC, 1–1(Z)-hexadecenyl-2-arachidonoyl-PC or 1-O-hexadecyl-2-eicosapentaenoyl-PC increased myotube diameter and myokine Il-15 mRNA expression. These results demonstrate a novel functionality of SPC and highlight the role of ether-type PC molecules in muscle function.

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鉴定从海星Patiria pectinifera中提取的用于C2C12肌管生长的活性EPA/AA结合醚型磷脂酰胆碱

人们越来越关注促进骨骼肌质量和功能的营养方法。本研究评估了海星衍生的甘油磷脂（PL）（SPL）对骨骼肌功能的影响，重点是 C2C12 肌细胞中肌管的形成。SPL是从Patiria pectinifera中通过氯仿/甲醇提取制备的，然后进行硅胶色谱分馏。无论是否经过 SPL 处理，都会诱导肌细胞分化。在分化的第 7 天，50 μg/mL 的 SPL 处理可增加肌管直径。磷脂酰胆碱（PC）部分（SPC）在30 μg/mL时也能促进肌管生长。LC-MS/MS分析表明，SPC中最丰富的PC分子种类是Alk-和Pls-PC，以及二十碳五烯酸和花生四烯酸。用 1-O-hexadecyl-2-arachidonoyl-PC, 1-1(Z)-hexadecenyl-2-arachidonoyl-PC 或 1-O-hexadecyl-2-eicosapentaenoyl-PC 处理可增加肌管直径和肌动素 Il-15 mRNA 的表达。这些结果证明了 SPC 的新功能，并强调了醚型 PC 分子在肌肉功能中的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Electronic Materials Multiple-

CiteScore

7.20

自引率

4.30%

发文量

567